α-胰凝乳蛋白酶在不同介质中稳定性的研究  

Stability of α-Chymotrypsin in Different Media

作　　者：沈鸿雁[1] 周闯[1] 叶蕴华[1] 田桂玲[1] 

机构地区：[1]北京大学化学与分子工程学院,教育部生物有机重点实验室,北京100871

出　　处：《北京大学学报（自然科学版）》2004年第4期578-582,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金项目 (2 0 372 0 0 8)

摘　　要：测定了α 胰凝乳蛋白酶在不同介质中的活性及稳定性。研究了有机溶剂性质、pH值、离子强度及离子类型对α 胰凝乳蛋白酶活性与稳定性的影响 ,并通过CD谱初步研究了α 胰凝乳蛋白酶在不同pH值缓冲液中的二级结构变化。研究结果表明 ,α 胰凝乳蛋白酶在某些有机溶剂中能在较长时间内维持较高的酶活性。α 胰凝乳蛋白酶在 3种pH值不同的缓冲液中的CD谱表明其在pH =1 0 0的稀溶液中的α 螺旋和 (或 ) β 折叠含量较高。在不同pH值条件下 ,离子强度对αThe activity and stability of α chymotrypsin in different media were studied. The influence of organic solvents, pH values, ionic strengths and ionic types on the stability of α chymotrypsin was preliminarily studied. The secondary structure of α chymotrypsin in different pH was studied by circular dichroism (CD) spectra. The results showed that α chymotrypsin could maintain its high activity and stability in some organic solvents for a long time. And the CD spectra of α chymotrypsin in solutions with different pH showed that in pH 10 0, the content of α helix and (or) β sheet of α chymotrypsin was higher than that in pH 5.0 or pH 7.0. The influence of ionic strengths on the activity and stability of α chymotrypsin was different in different pH values.

关 键 词：α-胰凝乳蛋白酶 稳定性 PH值 离子强度 CD谱 

分 类 号：O629[理学—有机化学]