自体血脑内注射建立大鼠脑出血模型实验研究  

Experimental study of rat cerebral hemorrhage model via autologous blood injection

作　　者：程小燕[1] 王红云[1] 安沂华[2] 

机构地区：[1]北京市神经外科研究所,北京市100050 [2]北京市神经外科研究所神经干细胞室

出　　处：《中国康复理论与实践》2004年第6期346-347,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市科技新星计划项目 (H0 2 0 82 1 360 1 30 ) ;北京市科委国际科技合作项目 (2 0 0 30 1 2 )

摘　　要：目的观察自体血脑内注射建立大鼠脑出血模型的效果。方法从大鼠心室腔内抽取血液 ,经立体定向注射到右侧尾状核内 ;通过脑组织病理切片、免疫组化、超微结构观察和运动功能检测等方法验证模型制作的可行性。结果术后大鼠即表现左侧肢体偏瘫 ;病理学检查证实右侧尾状核区血肿形成 ,且不同个体间血肿体积和部位的差异较小 ;透射电镜显示右侧内囊区广泛髓鞘变性 ;运动功能检测显示左侧肢体运动功能障碍 ,且个体间差异小于脑内胶原酶注射法 (P <0 .0 5 )。结论自体血脑内注射建立的大鼠脑出血模型 ,个体间血肿体积和部位、运动功能障碍程度的差异均小于脑内胶原酶注射法。ObjectiveTo observe the effect of rat cerebral hemorrhage model via autologous blood injection.MethodsBlood extracted from rats' cardiac ventricles was injected into the right caudate nucleus via stereotactic method. Pathological examination,ultrastructural visualization and motor functional test were applied to validate the feasibility of this method.ResultsRats showed paralysis of left extremities rapidly after surgery. Pathological examination confirmed the formation of hematoma in caudate nucleus. The deviation of hematoma volume and location among individuals was significantly lower than that of rats made by collagenase injection. Transmission electron microscopy showed wide mylin degeneration in right caudate nucleus. Motor functional test revealed the dysfunction of left extremities with marked lower deviation among individuals compared with that of collagenase injection ( P<0.05).ConclusionAutologous blood injection can result in significantly lower deviation of hematoma volume and location as well as dysfunctional degree compared with that of collagenase injection.

关 键 词：脑出血 中枢神经系统功能障碍 动物模型 超微结构 

分 类 号：R743.34[医药卫生—神经病学与精神病学]