胃癌细胞NF-κB和survivin的表达对TRAIL抗瘤作用的影响  

Effects of expressions of NF-κB and survivin on anti-tumor activity of TRAIL in gastric carcinoma cells

作　　者：杨柳芹[1] 房殿春[1] 

机构地区：[1]第三军医大学西南医院全军消化内科中心,重庆400038

出　　处：《第三军医大学学报》2004年第7期584-586,共3页Journal of Third Military Medical University

基　　金：全军"十五"科研基金资助项目 ( 0 1MA172 )~~

摘　　要：目的　探讨胃癌细胞NF κB和survivin的表达对TRAIL抗瘤作用的影响。方法　应用细胞培养和流式细胞仪检测的方法 ,观察胃癌细胞SGC 790 1、MKN2 8、AGS、MKN45经TRAIL作用后细胞凋亡率 ,Westernblot方法分析四种胃癌细胞中NF κB和survivin的表达。结果　TRAIL3 0 0ng ml作用于胃癌细胞 2 4h后 ,胃癌细胞MKN2 8、MKN45、AGS和SGC 790 1的凋亡率分别为 3 6 0 5 %、2 0 2 7%、16 5 0 %和 11 80 %。Westernblot结果显示SGC 790 1细胞NF κB、survivin的表达均高于MKN2 8细胞的表达。结论　TRAIL具有选择性诱导肿瘤细胞凋亡的特性 ,肿瘤细胞对TRAIL的耐药现象 ,可能与凋亡抑制因子survivin和NF κB的表达有关。Objective To investigate the effects of expressions of NF κB and survivin on the anti tumor activity of TRAIL in gastric carcinoma cells. Methods Gastric carcinoma cells were cultured in RPMI 1640. The apoptotic rates of gastric carcinoma cells SGC 7901, MKN28, AGS, and MKN45 after treatment with TRAIL were analyzed by flow cytometry. The expressions of NF κB and survivin in the 4 gastric carcinoma cells were determined by Western blotting. Results At 24 h after gastric carcinoma cells exposed to TRAIL at the dose of 300 ng/mL, the apoptosis percent ages were 36 05% for MKN28, 20 27% for MKN45, 16 50% for AGS, and 11 80% for SGC 7901. Western blotting showed that expressions of NF κB and survivin were lower in MKN28 than those in MKN45, AGS and SGC 7901. Conclusion The anti tumor sensitivity of TRAIL to gastric carcinoma cells is related to the expressions of NF κB and survivin.

关 键 词：胃癌 癌细胞 TRAIL 细胞凋亡 NF-ΚB SURVIVIN 

分 类 号：R735.2[医药卫生—肿瘤]