沙土鼠全脑梯度再灌注的脑保护实验研究  

Mechanism study of global cerebral protection for gerbil graded reperfusion

作　　者：张庆丰 董斌[2] 邱永明[2] 罗其中[2] 江基尧[2] 徐英辉[2] 

机构地区：[1]大连中心医院头颈外科,辽宁大连116033 [2]上海第二医科大学仁济医院神经外科,上海200001

出　　处：《中国微侵袭神经外科杂志》2004年第7期307-309,317,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金资助项目(39900153);上海市科委基金资助项目(00JC14046)

摘　　要：目的动态研究不同灌注时间和灌注量全脑组织缺血损害的恢复程度和即早基因的表达,以探明梯度再灌注的脑保护作用机制。方法将沙土鼠随机分为4组:实验组夹闭两侧颈总动脉,造成沙土鼠全脑缺血模型,夹闭10min后开放10min,开放不同脑血流量(1/4开放组,1/2开放组,一次性全开放组),分别检测3组及单纯血液稀释组缺血海马CA区C-fos蛋白的表达,以及缺血区大脑半球的改善状况。结果开放10min、1/2脑血流量时C-fos蛋白表达最高(P <0.05),海马CA区缺血损害改善最明显;开放10min、全脑血流量一次性开放时海马CA区损害最严重。结论①在梯度再灌注流量中,脑缺血海马区C-fos的表达和神经元凋亡呈反相作用关系;②低流量灌注有明显改善脑缺血的作用;③夹闭10min后,开放1/2脑血流量,持续10min的效果比一次性再灌注开放效果要好。Objective To evaluate the dynamic role of graded reperfusion (GR) to the brain and the relationship between GR and apoptotic genes expression after global ischemic insults, and the difference between the whole reperfusion and GR. Methods The gerbil global ischemic models were made by 10 min occlusion of bilateral carotid artery. Then the study models were randomized into four groups, the C-fos gene expression of the 10min reperfusion with different reperfusion flow (1/4, 1/2, full), and morphological results of hippocampus CA1 region were observed. Results C-fos expression was highly localized to the selectively vulnerable CA1 neuronal layer after moderate ischemia. 10min reperfusion, 1/2 reperfusion flow was the best way to increase C-fos expression and improved ischemic insults. Conclusions ①GR effectively increases C-fos expression of hippocampus CA region, and has antiapoptosis role. ②Low reperfusion flow improves ischemic insults. ③The results indicate that 10min, 1/2 reperfusion flow is better than full-open reperfusion ways.

关 键 词：梯度再灌注 C-FOS 凋亡 脑保护 

分 类 号：R363[医药卫生—病理学]