用自制酶电极测定牛奶中残留青霉素  

Determination of Trace Penicillin in Milk by Home-made Enzyme Electrode

作　　者：刘平[1] 李玉光[1] 初一鸣[1] 马洁[1] 薛颖[2] 吴国华[2] 刘梦友[1] 贾京津[1] 

机构地区：[1]首都师范大学化学系,北京100037 [2]北京市疾病预防控制中心

出　　处：《应用化学》2004年第8期832-834,共3页Chinese Journal of Applied Chemistry

基　　金：北京市教委资助项目 ( 2 0 0 2KJ0 94)

摘　　要：Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was determined by potentionmetric method. At low concentrations, the response time of the electrode modified by the method of crossing-link was 30～60 s, and the electrode showed high sensitivity. Linear detection range from 1.40×10 -3 to 3.36×10 -2 mmol/L in PBS and from 5.61×10 -2 to 3.36× 10 -1 mmol/L in milk were achieved. The electrode can be used for at least 7 d with perfect reproducibility during the first 4 d. The life of the electrode was 30 d.Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was determined by potentionmetric method. At low concentrations, the response time of the electrode modified by the method of crossing-link was 30～60 s, and the electrode showed high sensitivity. Linear detection range from 1.40×10 -3 to 3.36×10 -2 mmol/L in PBS and from 5.61×10 -2 to 3.36× 10 -1 mmol/L in milk were achieved. The electrode can be used for at least 7 d with perfect reproducibility during the first 4 d. The life of the electrode was 30 d.

关 键 词：生物电化学传感器 酶电极 青霉素残留 

分 类 号：O646[理学—物理化学] Q599[理学—化学]