一组高度保守的水稻trs-like基因的鉴定与分析  

Identification and Analysis of a Group of Highly Conserved trs-like Genes in Rice

作　　者：胡欣[1] 胡昊[1] 洪国藩[1] 韩斌[1] 

机构地区：[1]中国科学院上海生命科学研究院国家基因研究中心,上海200233

出　　处：《Acta Genetica Sinica》2004年第8期822-829,共8页

基　　金：中国科技部;中科院;上海市科委共同资助~~

摘　　要：基于对水稻基因组序列的注解和同源搜索的结果 ,用RT PCR结合测序的方法证明了水稻中至少有 10个具有转录活性的trs like基因。这 10个基因的编码产物与酵母TRAPP蛋白复合体已知 10个亚基中的 6个分别同源。其中 4对基因是双拷贝的 ,另 2个则是单拷贝的 (基于已知的水稻基因组序列 )。所有这 10个基因均在不同时期的水稻组织中广泛表达 ,并与其他真核生物的trsThere are at least ten transcriptional trs-like genes in rice that have been confirmed by RT-PCR and sequencing, based on the annotation results of rice genome and homologous search. These ten genes correspond to six of the ten known subunits of TRAPP complex in yeast. Four pairs of them are duplicates while the other two are unique according to the known rice genomic sequences. All of the ten genes are constitutively expressed in rice tissues and share phylogenetic homology to some extent with other eukaryotic trs-like genes in their gene structures and protein sequences.

关 键 词：水稻 TRAPP亚基 内质网-高尔基体转运牵引 基因组序列注解 反转录PCR 

分 类 号：S511[农业科学—作物学]