大鼠再生肝抗四氯化碳损伤作用——粗面内质网的形态计量学研究  

ANTAGONISTIC EFFECTS OF REGENERATED LIVER AGAINST CCl_4 IN RATS——A STUDY OF ELECTRON MICROSCOPIC MORPHOMETRY ON ROUGH ENDOPLASMIC RETICULUM

作　　者：程时[1] 侯琳[1] 贾鸿宴 李伟路 毕振伍 

机构地区：[1]北京医科大学,北京100083

出　　处：《动物学报》1993年第4期412-417,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(389T0414)

摘　　要：电镜形态计量学证明,大鼠再生肝对CCl_4损伤有明显抵抗作用。根据肝小叶外带肝细胞体密度、粗面内质网面密度、比表面及单位膜面附着核糖体数等参量的测算,再生肝接受CCl_4后肝细胞损伤减轻,粗面内质网不肿胀,膜面积及附着的核糖体数比正常肝CCl_4损伤后的相应数值增加。假手术动物对CCl_4肝损伤也有抵抗作用。这种细胞保护机制可能与应激后肝细胞内金属硫蛋白合成增加有关。Partial hepatectomy in rats was carried out by the method of Higgins and Andarson, the pseudooperated (laparotomy only, without hepatectomy)and intact animals were used as controls. All the rats were treated with CCI4. The protective effects of regenerated liver against CCl4 were shown by means of electron microscopic morphometry. The parameters, volume density (Vv) of liver parenchymal cell components, surface density (Sv) ,and specific surface (δ) of rough endo plasmic reticulum (RER)and the number of attached ribosomes per unit membrane (Ns)of RER in parenchymal cells at peripheral zones were measured and calculated. Although there was no obvious difference in Vv between injured group and the group of regenerated liver, the values of Sv and 6 of RER in the group of regenerated liver and pseudooperated group were hlgher than those of injured group. N. was higher in the first two groups than that of the last one as well. The results showed that after hepatectomy, in liver parenchymal cells of rats treated with CCl4, the membrane area of RER, did not reduced, and lumen of RER was not expanded. Suggesting RER, one of the attacking targets of CCl4,did not obviously injured. The livers of rats which Subjected either to hepatectomy or to psudooperation showed resistence to CCl4. The mechanism pf cell protection against CCl4 is associated with the increase in metallothionein synthisis in liver following stress.

关 键 词：再生肝 粗面内质网 四氯化碳 损伤 

分 类 号：R363[医药卫生—病理学]