Inhibition of Fas/FasL mRNA expression and TNF-a release in concanavalin A-induced liver injury in mice by bicyclol  

作　　者：MinLi Geng-TaoLiu 

机构地区：[1]DepartmentofPharmacology,InstituteofMateriaMedicaandPekingUnionMedicalCollegeandChineseAcademyofMedicalSciences,Beijing100050,China

出　　处：《World Journal of Gastroenterology》2004年第12期1775-1779,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the Grant from Ministry of Science and Technology of China,No.94-ZD-02

摘　　要：AIM: Bicyclol, 4,4'-dimethoxy-5,6,5',6'-dimethylene-dioxy-2-hydroxymethy1-2'-carbonyl biphenyl, is a new anti-hepatitis drug. The aim of the present study was to investigate the protective effect of bicyclol on concanavalin A (Con A)-induced immunological liver injury in mice and its mechanism. METHODS: Liver injury was induced by injection of Con A via tail vein of mice and assessed biochemically and histologically. Serum transaminase and tumor necrosis factor alpha (TNF-a were determined. Liver lesions were observed by light microscope. Expressions of TNF-a, interferon gamma (IFN-y), Fas and Fas ligand (FasL) mRNA in the livers were measured by RT-PCR. RESULTS: Serum transaminase level and liver lesions in Con A-induced mice were markedly reduced by oral administration of 100, 200 mg/kg of bicyclol. TNF-a level inserum was also reduced by bicyclol. Con A injection in ducedup-regulation of TNF-a, IFN-7, Fas and FasL mRNA expression in liver tissues. Bicyclol significantly down-regulated the expression of IFN-y, Fas and FasL mRNA, but only slightly affected TNF-a mRNA expression in liver tissues. CONCLUSION: Bicyclol protects against Con A-induced liver injury mainly through inhibition of Fas/FasL mRNA expression in liver tissues and TNF-a release in mice.AIM:Bicyclol,4,4'-dimethoxy-5,6,5',6'-dimethylene-dioxy- 2-hydroxymethyl-2'-carbonyl biphenyl,is a new anti-hepatitis drug.The aim of the present study was to investigate the protective effect of bicyclol on concanavalin A(Con A)-induced immunological liver injury in mice and its mechanism. METHODS:Liver injury was induced by injection of Con A via tail vein of mice and assessed biochemically and histologically.Serum transaminase and tumor necrosis factor alpha(TNF-α)were determined.Liver lesions were observed by light microscope.Expressions of TNF-α,interferon gamma (IFN-γ),Fas and Fas ligand(FasL)mRNA in the livers were measured by RT-PCR. RESULTS:Serum transaminase level and liver lesions in Con A-induced mice were markedly reduced by oral administration of 100,200 mg/kg of bicyclol.TNF-α level in serum was also reduced by bicyclol.Con A injection induced up-regulation of TNF-α,IFN-γ,Fas and FasL mRNA expression in liver tissues.Bicyclol significantly down-regulated the expression of IFN-γ,Fas and FasL mRNA,but only slightly affected TNF-α mRNA expression in liver tissues. CONCLUSION:Bicyclol protects against Con A-induced liver injury mainly through inhibition of Fas/FasL mRNA expression in liver tissues and TNF-α release in mice.

关 键 词：抑制剂 FAS/FASL mRNA 基因表达 TNF-α 伴刀豆蛋白 A-诱导 肝损害 老鼠 免疫学 

分 类 号：R575[医药卫生—消化系统]