TRAIL基因与鸡贫血病毒vp3基因体外协同效应的研究  

Hepatoma Cell Apoptosis-inducing Effect of TRAIL Cooperates With vp3 In Vitro

作　　者：彭冬君[1] 尹燕华[1] 王宇哲[1] 孙军[1] 宗义强[1] 屈伸[1] 

机构地区：[1]华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市430030

出　　处：《医学分子生物学杂志》2004年第2期94-97,共4页Journal of Medical Molecular Biology

摘　　要：目的研究TRAIL基因与鸡贫血病毒vp3基因在体外协同诱导肝癌细胞系HepG2凋亡的作用。方法从人外周血淋巴细胞总RNA中扩增出TRAIL基因的cDNA序列,构建含TRAIL基因的真核表达重组体,与鸡贫血病毒vp3基因在体外共转染HepG2细胞,研究其协同诱导HepG2细胞凋亡作用。结果 单独转染TRAIL基因与vp3基因均可诱导HepG2细胞凋亡;将两种基因共转染HepG2细胞,发现其凋亡率明显增加。结论TRAIL基因与vp3基因在诱导HepG2细胞凋亡中存在正协同效应。Objective To explore the hepatoma cell line HepG2 apoptosis-induced effect of TRAIL cooperating with vp3 in vitro. Methods Eukaryotic expression vector for TRAIL cDNA acquired from human lymphocytes was constructed. After co-transfecting with vp3 gene, HepG2 apoptosis-induced effect was observed. Results TRAIL and vp3 gene could induce apoptosis of HepG2 cells in vitro, and enhanced effect was observed by co-transfecting TRAIL with vp3 gene. Conclusion Positive-cooperative effect of TRAIL and vp3 exist in apoptosis-induction of HepG2 cells.

关 键 词：肝脏肿瘤 凋亡 TRAIL基因 VP3基因 

分 类 号：R735.7[医药卫生—肿瘤]