FAK反义寡聚脱氧核苷酸对FN诱导Hela细胞MMP-2表达的影响  

EFFECT OF FAK ANTISENSE ODN ON THE EXPRESSION OF MMP-2 INDUCED BY FN TO HELA CELL

作　　者：班莺[1] 于晓光[1] 赵丹丹[1] 林平[1] 徐华锋[1] 邹海峰[1] 刘洋[1] 

机构地区：[1]哈尔滨医科大学生物化学与分子生物学教研室,黑龙江哈尔滨150086

出　　处：《肿瘤研究与临床》2004年第3期150-152,共3页Cancer Research and Clinic

基　　金：黑龙江省高校骨干教师创新能力资助课题(2002)

摘　　要：目的:利用反义核酸技术探讨纤黏连蛋白(fibronectin, FN)诱导Hela细胞中基质金属蛋白酶(matrix metalloproteinases, MMPs)基因表达的机制。方法:无血清培养Hela细胞,以不同浓度及作用时间的FN诱导Hela细胞中MMPs基因表达, 并用反义寡聚脱氧核苷酸(oligodeoxynucleotides,ODN)封闭焦点黏着激酶(focal adhesion kinase,FAK), 用酶谱分析方法检测MMPs的活性。结果:FN浓度为0 μg/mL时,Hela细胞无MMP蛳2基因表达;FN浓度为5 μg/mL^10 μg/mL、作用时间为12 h,MMP蛳2活性最大;当在培养液反义ODN后,MMP蛳2活性明显降低。结论:MMP蛳2基因表达与FN的浓度以及作用时间有关;FN可通过其整合素受体的信号转导通路启动Hela细胞中MMP蛳2基因表达、降解ECM,从而促进肿瘤的浸润和转移。Objective: To study the mechanism of the expression of MMPs gene in Hela cell induced by FN through antisense oligonucleotides technology. Methods: Hela cell cultured in RPIM1640 without calf serum, by different concentration and different time of FN induced the expression MMPs gene, and antisense ODN was used to block FAK, gelatin zymography was used to measure the activity of MMPs. Results: When the concentration of FN got to 0 μg/mL, there was no activity of MMP- 2; when the concentration of FN got to 5 μg/mL^10 μg/mL after 12 hours, the activity of MMP- 2 was the highest; the activity of MMP- 2 was dramatically decreased when antisense ODN was put into the culture medium. Conclusion : There was relationship between the activity of MMP- 2 and the concentration and the time of FN. Through the signal transduction of intergrin receptor , FN enhanced the expression of MMP- 2 and accelerated the transfer of tumor.

关 键 词：寡聚脱氧核苷酸 纤黏连蛋白 基质金属蛋白酶 焦点黏着激酶 

分 类 号：R73-354[医药卫生—肿瘤]