人内皮抑素基因转染对舌鳞癌Tca8113细胞生长的影响  

The inhibitory effect of human endostatin gene on tumor growth of tongue squamous cell carcinoma

作　　者：潘朝斌[1] 黄洪章[1] 王建广[1] 侯劲松[1] 李海刚[1] 

机构地区：[1]中山大学附属第二医院口腔颌面外科,广州510120

出　　处：《中华口腔医学杂志》2004年第4期273-276,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金(30271423);广东省医学科学技术研究基金(2003A205)资助项目

摘　　要：目的 探讨人内皮抑素(hES)基因导入舌鳞癌Tca8113细胞后的表达情况及其抑制肿瘤生长效应。方法 以脂质体为载体将hES基因导入Tca8113细胞,通过动物实验观察转基因Tca8113细胞移植瘤生长情况,免疫组织化学检测肿瘤组织中hES及血管内皮细胞生长因子(VEGF)的表达,Weidner法行肿瘤微血管密度(MVD)计数,流式细胞仪检测肿瘤细胞凋亡率。结果 移植瘤生长第27天,实验组肿瘤组织中hES的表达率高于对照组(P<0.01);VEGF的表达率低于对照组(P<0.01)。实验组MVD计数低于对照组(P<0.01),而肿瘤细胞凋亡率则明显高于对照组(P<0.01)。hES基因转染对肿瘤生长的抑制率78.9％。结论 hES基因转染舌鳞癌细胞可体内诱导hES蛋白高效表达并具有抑制移植瘤生长的效应。Objective To investigate the expression of transfected human endostatin (hES) gene in tongue squamous cell carcinoma (TSCC) and its inhibitory effects on the growth of tumor cells in vivo. Methods Lipofectamine-mediated hES gene was transferred into Tca8113 cells, selected with Blasticidin S; The stable trancfected cells were inoculated in BALB/c mice, and then the growth of xenografts was observed. The hES and vascular endothelial growth factor ( VEGF) protein expression of xenografts was detected by S-P immuno-histochemical assay. We also detected the microvessel density ( MVD) of xenografts with Weidern's method and apoptotic index of the tumor cells by flow cytometry (FCM). Results The hES protein expression of xenografts in experimental group was significantly higher than that in control group (P <0. 01) , while the expression of VEGF protein was on the other way round (P <0. 01). MVD counting of xenografts in experimental group was lower than that in control group ( P < 0. 01 ). The mean apoptotic level of the tumor cells in control group was also lower than in experimental group (P <0. 01). In addition, the inhibitory rate to growth of xenografts induced by hES transfection was 78. 9% . Conclusions hES gene can be transferred into TSCC cells and then induce corresponding protein expression efficiently in xenograft model, resulting in significantly inhibitory effects on the xenografts in vivo.

关 键 词：内皮抑素 基因转染 舌鳞癌 TCA8113细胞 肿瘤生长 

分 类 号：R739.8[医药卫生—肿瘤]