米诺环素对体外培养的人牙周韧带细胞生物活性的影响  

Effects of minocycline on biobehavior of human penodontal ligament cells in vitro

作　　者：葛少华[1] 杨丕山[1] 赵宁[1] 戚向敏[1] 孙钦峰[1] 王艳[1] 

机构地区：[1]山东大学口腔医学院牙周黏膜病科,济南250012

出　　处：《中华口腔医学杂志》2004年第4期284-286,共3页Chinese Journal of Stomatology

摘　　要：目的 研究米诺环素对体外培养的人牙周韧带细胞(HPDLCs)增殖及生物合成的影响。方法 将不同浓度的米诺环素(1、5、20、100、500、2500 mg/L)加入体外培养的HPDLCs,共孵育2d后,在倒置显微镜下观察其对细胞形态的影响,并分别检测其对细胞的增殖活性、蛋白合成及DNA合成的影响。结果 在5-100mg/L的浓度范围内,米诺环素可显著促进HPDLCs的增殖及生物合成(P<0.01)。但高浓度(2500 mg/L)的米诺环素则严重抑制细胞的生物学活性。结论 一定浓度的米诺环素能提高HPDLCs的生物学活性,但过高浓度的米诺环素具有细胞毒性,不利于牙周组织的修复和再生。Objective To evaluate the influence of minocycline on the proliferation and biosynthesis of human periodontal ligament cells (HPDLCs) in vitro. Methods Various concentrations of minocycline (1,5,20,100,500,2500 mg/L)were added to the medium of cultured HPDLCs respectively. After co-incubated for 2 days, cell morphology was observed under reverse microscope, cell proliferation activity was assayed using MTT, the total amount of protein was detected with Coumassie Bright Blue method and DNA synthesis was measured by H-TdR. Results The presence of minocycline not exceeding 500 mg/L in the medium resulted in no morphological change of HPDLCs. Moreover, at a concentration range of 5 to 100 mg/L, minocycline significantly enhanced the proliferative activity and biosynthesis of HPDLCs (P < 0. 01 ). However, higher concentration (2500 mg/L) not only changed cell morphology under microscope, but also significantly inhibited cellular activity. Conclusions The results suggest that proper doses of minocycline could promote biobehavior of HPDLCs, while higher concentrations of minocycline had cytotoxic effect which may intervene affect tissue repair and regeneration.

关 键 词：米诺环素 体外培养 人牙周韧带细胞 生物活性 细胞增殖 生物合成 四环素 

分 类 号：R781.4[医药卫生—口腔医学]