颞下颌关节骨关节病髁突软骨细胞及基质合成代谢特性的研究  

Study on the metabolism of cartilage matrix by the chondrocytes in osteoarthritic condylar cartilage

作　　者：常嘉[1] 马绪臣[1] 马大龙[2] 李现庭[2] 夏东岚[2] 

机构地区：[1]北京大学口腔医学院颞下颌关节病及口颌面痛诊治中心,100081 [2]北京大学医学部分子免疫学实验室

出　　处：《中华口腔医学杂志》2004年第4期309-312,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目(30171015)

摘　　要：目的 探讨颞下颌关节骨关节病(TMJOA)软骨细胞的细胞生物学特性。方法 通过手术切除部分关节盘的方法,制造TMJOA的兔动物模型;从模型动物处于骨关节病病变增殖修复期的髁突软骨组织中获取细胞体外培养,并采用RT-PCR法比较病变软骨细胞与正常对照细胞对软骨基质蛋白、基质胶原酶和内源性生长因子的表达差异。结果 从TMJOA模型的髁突软骨组织中获得细胞经体外培养、鉴定,确认为软骨细胞;骨关节病髁突软骨细胞对软骨基质蛋白的表达不平衡,对内源性基质胶原酶的表达水平和内源性生长因子TGF-β1、IGF-1的表达明显增强。结论 本研究从细胞生物学角度证实了骨关节病早期关节软骨内出现合成代谢活跃的组织学特点,并发现该阶段组织细胞的修复代偿反应可致软骨基质成分合成的不平衡,最终可导致软骨基质环境改变。Objective To study the characteristics of cellular metabolism of mandibular condylar chondrocytes in repairing state of osteoarthrosis and investigate its role in the pathogenesis of the disease. Methods Temporomandibular joint osteoarthrosis model of rabbits was created by the partial resection of joint disc and confirmed with histological diagnosis. The chondrocytes were harvested from osteoarthritic condylar cartilage in the repairing state and cultured in vitro under the monolayer culture condition. The cellular expression of cartilaginous matrix protein, collagenase and growth factors between the osteoarthritic chondrocytes and the normal controls were measured with RT-PCR technique to outline the basic feature of the osteoarthritic cells. Results The cultured cells were confirmed as chondrocytes with their ability of expression of collagen type Ⅱ and Aggrecan. In the reactive repairing state of osteoarthrosis, the chondrocytes showed the imbalance of expression of ECM proteins, and increased expression of collagenase and endogenous growth factors such as IGF-1 and TGF-β1. Conclusions This study found the active anabolism of the chondrocytes within the osteoarthritic condylar cartilage and the imbalance synthesis of cartilage matrix. These repairing attempts by the osteoarthritic chondrocytes may be impossible to restore the primary homeostasis within the condylar cartilage.

关 键 词：颞下颌关节骨关节病 软骨细胞 基质合成 基质代谢 下颌骨髁状突 基质胶原酶 内源性生长因子 

分 类 号：R782[医药卫生—口腔医学]