dbcAMP诱导的EoL-1分化和凋亡与bcl-2家族的关系  

The Relationship between the Differentiation and Apoptosis in EoL-1 Induced by dbcAMP and the bcl-2 Family

作　　者：台桂香[1] 刘静[2] 高素君[3] 

机构地区：[1]吉林大学基础医学院免疫教研室,长春市130021 [2]长春市儿童医院 [3]吉林大学第一医院血液科

出　　处：《中国肿瘤临床》2004年第13期729-731,共3页Chinese Journal of Clinical Oncology

基　　金：教育部归国留学人员启动基金资助(编号:9922G167)

摘　　要：目的:探讨dbcAMP(dbutyrylcyclicAMP)诱导的EoL-1(eosinophilicleukemiacellline)分化和凋亡与bcl-2家族的关系。方法:通过免疫印记和流式细胞仪调查bcl-2家族蛋白在dbcAMP刺激的EoL-1细胞中的表达。结果:EoL-1在正常培养条件下表达高水平的bcl-2、bcl-xL和中等水平的bax,但未表达bcl-xs。dbcAMP诱导了EoL-1细胞下调表达bcl-2和bax及上调表达bcl-xs。结论:dbcAMP诱导EoL-1分化和凋亡与bcl-2的降低以及bcl-xs的升高有关.Objective: To research the relationship between differentiation and apoptosis of EoL-1 induced by dbcAMP and bcl-2 family. Methods: We examined the bcl-2 family expression in EoL-1 cells cultured with dbcAMP by Western blot and flow cytometry. Results: We found that EoL-1cells under normal culture conditions expressed bcl-2, bcl-xL and bax at high level but not bcl-xs. dbcAMP downregulated bcl-2 and bax level but upregulated bcl-xs level. Conclusion: It surggests that the eosinophilic differentiation and apoptois in EoL-1 stimulated by dbcAMP partly have relations with the decrease of bcl-2 and increase of bcl-xs.

关 键 词：DBCAMP BCL-2家族 EoL-1凋亡 

分 类 号：R73-3[医药卫生—肿瘤]