应用RT-PCR检测乳腺癌患者外周血CEA mRNA和CK-19 mRNA的研究及意义  

Detection of CEA mRNA and CK-19 mRNA in Peripheral Blood from Breast Cancer Patients by Reverse Transcription Polymerase Chain Reaction

作　　者：关泉林[1] 谭继英[2] 汪晓伟[1] 高明太[3] 李玉民[3] 袁文臻[1] 

机构地区：[1]兰州医学院第一附属医院肿瘤外科,兰州市730000 [2]兰州医学院免疫学教研室 [3]兰州医学院第一附属医院肿普外科,兰州市730000

出　　处：《中国肿瘤临床》2004年第13期732-734,740,共4页Chinese Journal of Clinical Oncology

基　　金：甘肃省自然科学基金资助(编号:QS992-C33-023-14)

摘　　要：目的:检测乳腺癌患者外周血中CEAmRNA和CK-19mRNA的表达,建立取材于外周血早期诊断乳腺癌微转移的方法。方法:65例乳腺癌和37例乳腺良性疾病患者的外周血,分离其有核细胞后进行细胞总RNA的抽提,运用巢式RT-PCR技术进行CEAmRNA和CK-19mRNA的检测。结果:以巢式RT-PCR终产物出现131bp为CEAmRNA阳性,460bp为CK-19mRNA阳性。65例乳腺癌患者CEAmRNA和CK-19mRNA分别有26例和24例表达,总阳性率分别为40.0%和36.92%。37例乳腺良性疾病者无1例CEAmRNA和CK-19mRNA表达,两组有统计学意义(P<0.001)。结论:RT-PCR可以用于临床检测乳腺癌患者外周血中微转移。Objective: Detect carcinoembryonic antigen mRNA(CEA mRNA) and cytokeratin-19(CK-19mRNA) in nucleated cells in peripheral blood from breast cancer. To establish an early diagnostic method for breast cancer micrometastasis in peripheral blood. Methods: Peripheral blood samples in breast cancer patients (test group,n=65) and benign tumour in breast patients (control group, n=37) were taken. Then, separated nucleated cells and extracted total RNA, CEA mRNA and CK-19mRNA was detected by nested reverse transcription polymerase chain reaction(nested RT-PCR) technique. Results: Samples were diagnosed CEAmRNA positive when 131 bp band is appeared in nested RT-PCR end-product, CK-19mRNA is460bp as well. The positive rates of CEAmRNA and CK-19mRNA is, respectively 40% (26/65) and 36.92%(24/65) in test group. None benign tumor in breast patients express CEAmRNA and CK-19mRNA. The difference between the two groups was statistically significant (P<0.001). Conclusion: RT-PCR can be diagnosed in breast cancer micrometastasis, with relatively high sensitivity.

关 键 词：乳腺癌 外周血 微转移 RT-PCR CEA MRNA CK-19 MRNA 

分 类 号：R737.9[医药卫生—肿瘤]