测定脱氢酶活性的萃取剂选择  

Extractant Selection in Measuring Activity of Dehydrogenase

作　　者：尹军[1] 谭学军[2] 张立国[2] 付瑶[1] 

机构地区：[1]吉林建筑工程学院环境工程系,吉林长春130021 [2]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090

出　　处：《中国给水排水》2004年第7期96-98,共3页China Water & Wastewater

基　　金：吉林省科技发展计划项目(95280)

摘　　要：　选择最佳萃取剂是提高脱氢酶活性测定结果准确性的重要步骤之一,因此在测定内源呼吸TTC-脱氢酶活性时,选择了丙酮、乙醇、正丁醇、磷酸三丁酯、乙酸乙酯、甲苯和三氯甲烷七种萃取剂,就标准曲线、最佳萃取时间以及萃取污泥中三苯基甲酯(TF)的能力等三个方面进行了比较。综合考虑各种性能后确定丙酮为最佳萃取剂。Selection of optimal extractant was one of important measures for improving accuracy in measuring dehydrogenase activity,therefore seven kinds of extractant such as acetone,ethanol,n-butanol,tributyl phosphate,ethyl acetate,methylbenzene,and trichloromethane were selected for measuring endogenous TTC-dehydrogenase activity.Comparison was made in the regards of standard curve,the best extraction time,and extraction rate of TF from sludge.After overall consideration for all characteristics,acetone was determined to be the best extractant.

关 键 词：TTC-脱氢酶活性 内源呼吸 萃取剂 标准曲线 

分 类 号：X131[环境科学与工程—环境科学]