日本血吸虫(大陆株)成虫表达序列标签的获取及电子延伸  

Acquirement of the expressed sequence tag of Schistosoma japonicum (Chinese strain) and electronic-elongation

作　　者：邵筱[1] 余新炳[1] 吴忠道[1] 徐劲[1] 李焱[1] 刘翰腾[1] 李华文[1] 李宝华[1] 

机构地区：[1]中山大学基础医学院寄生虫学教研室,广州510089

出　　处：《中国地方病学杂志》2004年第4期297-299,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(30070682)

摘　　要：目的获取日本血吸虫(Schistosomajaponicum,Sj)新基因。方法从Sj(大陆株)成虫cDNA文库中随机挑选单个重组克隆进行部分测序以获取表达序列标签(expressedsequencetag,EST),用其提供的BLAST程序和Genebank数据库进行序列比较和同源性分析;建立电子拼接的方法,对所获EST进行电子延伸,并对Sj延伸后序列进行同源性分析。结果在Sj成虫cDNA文库中,随机挑选182个单个重组克隆,获取53个有价值EST序列,并在Genebank中登录,获得53个EST的延伸序列及分析结果。结论Sj表达基因EST测序、同源性分析及EST的电子延伸是获取Sj新基因的有效对策。Objective To research and analyze the constitutes of the genome of Schistosoma japonicum, and get those genes that be worth researching in future. Methods Selected single recombined clones stochastically and got sequences, namely expressed sequence tags(EST) from cDNA library of Schistosoma japonicum(Chinese strain); logged on the homepage of NCBI, then identified and analyzed the EST utilizing the procedure of BLAST and database of Genebank offered by NCBI; exercised the method of the Electronic-cDNA Cloning, elongated the sequences in order to learn more meritorious information, and analyzed the sequences elongated. Results Picked out 182 sole random clones and got 53 valuable EST that logged on the Genebank from cDNA library of Schistosoma japonicum(Chinese strain); on the other hand, obtained 44 sequences that had elongated and analyzed. Conclusions It can make the groundwork for the research of the functional genes for us to combine the sequencing, comparing of EST and the system of electronic-cDNA cloning.

关 键 词：日本血吸虫 电子延伸 表达序列标签 成虫 生物信息学 

分 类 号：R346[医药卫生—基础医学]