顺铂及拓扑替康对卵巢癌裸鼠移植瘤生长的抑制作用  

Anticancer effects of cisplatin and topotecan on ovarian cancer in nude mice

作　　者：史惠蓉[1] 侯丽娟[1] 乔玉环[1] 兰莉[1] 常青[1] 张钦宪[2] 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052 [2]郑州大学基础医学院组织学与胚胎学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第4期563-565,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技创新人才工程项目 2 0 0 3 0 2 7

摘　　要：目的 :了解顺铂 (DDP)、拓扑替康 (TPT)单独及联合应用对卵巢癌裸鼠皮下移植瘤生长的抑制作用。方法 :裸鼠右侧背部皮下接种人卵巢癌SKOV3细胞 ,2 1d后分 4组治疗 ,每组 6只动物。对照组 ,腹腔注射无菌生理盐水 ;DDP组 ,腹腔注射DDP 3mg·kg-1 ;TPT组 ,腹腔注射TPT 5mg·kg-1 ;DDP +TPT组 ,腹腔注射DDP 3mg·kg-1 ,6h后腹腔注射TPT 5mg·kg-1 ;上述药物均每 4d注射 1次 ,共注射 4次 ,于实验第 39d处死裸鼠。治疗期间观察并记录各组裸鼠皮下移植瘤的生长情况 ,计算肿瘤体积、肿瘤生长抑制率 ,绘制肿瘤生长曲线。结果 :各治疗组治疗后肿瘤体积和抑瘤率与对照组相比差异均具有统计学意义 (P <0 .0 5 )。与DDP及TPT组比较 ,DDP +TPT联合用药组肿瘤体积明显减小 (P <0 .0 0 1 ) ,抑瘤率明显增高 (P <0 .0 0 1 )。结论 :DDP与TPT联合用药比 2药单独应用抑制肿瘤生长的作用更强 。Aim: To evaluate the anticancer effects of cisplatin,topotecan, and cisplatin with topotecan to human ovarian epithelial cancer in nude mice. Methods: Twenty four nude mice were subcutaneously implanted with human ovarian epithelial cancer cell line SKOV3 on the right side of nude mice's back, and divided into 4 groups after 21 days:control group, cisplatin group, topotecan group,and cisplatin with topotecan group.Different treatments were served from 21st day after implantation and all mice were sacrificed after 39 days. The tumor volumes of the mice were measured respectively during the therapy time.The tumor growth inhibiting rates of each group were calculated. Moreover, the curves of tumor growth were drew. Results:Significant differences in average tumor volumes and tumor growth inhibition rates were found between the treatment group and the control group( P <0.05).The tumor volumes in combination group were significantly less than those of other groups( P <0.001). Compared with cisplatin group(26.54±8.42)% and topotecan group (38.18±11.12)%,the growth inhibiting rates of tumor in combination group (73.17±30.78)% were significantly higher. Conclusion: Combined application of cisplatin and topotecan has stronger inhibition on tumor growth than single use,and they has a synergic anticancer effect to ovarian cancer.

关 键 词：卵巢肿瘤 裸鼠 顺铂 拓扑替康 

分 类 号：R737.3[医药卫生—肿瘤]