拓扑替康和更昔洛韦单独或联合应用对SKOV3/tk细胞生长的影响  

Effect of ganciclorir and topotecan on SKOV3/tk cells

作　　者：郭玉忠[1] 史惠蓉[2] 姜国忠[1] 李克虹[2] 王建民[1] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052 [2]郑州大学第一附属医院妇产科,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第4期569-571,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省重大科技攻关基金资助项目 0 12 2 0 3 2 5 0 0

摘　　要：目的 :探讨更昔洛韦 (GCV)与拓扑替康 (TPT)单独或联合应用对携带有hTERT启动子调控的HSV tk基因的SKOV3细胞生长的影响。方法 :将hTERT启动子调控的HSV tk基因重组逆转录病毒载体成功转染人卵巢癌SKOV3细胞 (SKOV3/tk细胞 ) ,用MTT法观察SKOV3/tk细胞对GCV、TPT的敏感性 ;观察合用GCV和TPT对SKOV3/tk细胞的杀伤作用。结果GCV及TPT对SKOV3/tk细胞的最低有效浓度分别为 1 0mg/L和 1 0 μg/L ,1 0 0mg/LGCV与TPT合用时TPT对SKOV3/tk细胞的最低有效浓度为 5 μg/L。 结论 :GCV合用TPT较TPT单用更能抑制携带有HSVAim: To investigate the effect of ganciclovir and topotecan on SKOV3 cell transfected HSV tk under the control of hTERT promoter. Methods: The recombinant vectors carrying hTERT promoter and HSV tk gene was constructed and transfected into SKOV3 cells. The sensitivity of the transfected cells to GCV, topotecan, GCV with topotecan were observed using MTT method, respectively. Results:The growth of the transfected cells was greatly inhibited by GCV administration at 10 mg·L -1 and topotecan at 10 μg·L -1 . Topotecan dosage could be decreased to 5 μg·L -1 when combined with GCV 100 mg·L -1 .Conclusion: The growth of SKOV3 cells transfected HSV tk gene under the control of hTERT promoter could be greatly inhibited by GCV, and the GCV with topotecan combination shows high efficiency in killing the cells.

关 键 词：HTERT启动子 HSV-TK基因 卵巢癌细胞 拓扑替康 更昔洛韦 

分 类 号：R979.1[医药卫生—药品]