渗透浓度对肝癌细胞增殖的影响  

Effects of culture medium osmolarity on the proliferation of human hepatoma cells

作　　者：田晶[1] 王瑜[2] 佟文革[1] 张宸豪[1] 

机构地区：[1]第四军医大学吉林军医学院生理教研室,吉林省吉林市132013 [2]第四军医大学吉林军医学院临床检验教研室,吉林省吉林市132013

出　　处：《医学研究生学报》2004年第7期584-587,共4页Journal of Medical Postgraduates

摘　　要：目的 :观察渗透浓度对人肝细胞肝癌 (HHCC)细胞系增殖的影响 ,为容积调控氯离子通道参与细胞增殖提供进一步证据。　方法 :改变培养液的渗透浓度 ,应用噻唑蓝比色分析法及细胞计数法观察细胞增殖情况 ;流式细胞仪检测细胞周期时相。　结果 :细胞在低渗培养液中增殖旺盛 ,在高渗培养液中增殖减慢 (P <0 .0 1 )。经高渗处理 4 8h的HHCC细胞G1 期细胞比例比对照组明显增高 ,S期及G2 期细胞比例明显低于对照组 (P <0 .0 5 ) ,而经低渗处理 4 8h的细胞 ,G1 期细胞比例明显降低 ,S期及G2 期细胞比例明显高于对照组 ,并且低渗对细胞周期的影响可被氯离子通道阻断剂尼氟灭酸 (NFA)减弱 (P <0 .0 5 )。　结论 :渗透浓度可以通过影响细胞增殖周期而影响细胞增殖。Objective: To investigate the effects of culture medium osmolarity on the proliferation of human hepatoma cell line HHCC. Methods:Cell proliferation was analyzed by MTT assay and cell cycle analysis was carried out by flow cytometry. Results:Hyperosmolarity decreased significantly the absorbance (A) of HHCC cells and cell number, and hyposmolarity have counter effects. Compared with control, hyperosmolarity blocked cell cycle progression at the G 1 phase, while hyposmolarity accelerated cells into S phase from G 1 phase, and the effect of hyposmolarity was weakened by chloride channel blocker NFA. Conclusion:Culture medium osmolarity influenced the proliferation of HHCC cells by mediating cell cycle.

关 键 词：渗透浓度 肝癌 增殖 

分 类 号：Q253[生物学—细胞生物学] R735.7[医药卫生—肿瘤]