γ-氨酪酸和谷氨酸与胃癌关系的研究  

Relationship between GABA,Glu and gastric cancer

作　　者：秦苏堤[1] 朱人敏[1] 何小平[1] 汪芳裕[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院消化内科,江苏南京210002

出　　处：《医学研究生学报》2004年第7期620-623,共4页Journal of Medical Postgraduates

摘　　要：目的 :探讨γ 氨酪酸 (GABA)和谷氨酸 (Glu)在胃癌组织、正常胃黏膜组织含量的差异。　方法 :采用高效液相色谱法 ,用异硫氰酸苯酯 (PITC)作衍生剂 ,紫外检测波长 2 5 4nm ,检测 30例胃癌组织、正常胃黏膜组织中GABA和Glu含量。　结果 :与正常胃黏膜组织比较 ,胃癌组织中GABA和Glu含量明显增加 (P <0 .0 5 ) ,但与胃癌的组织分型及淋巴结转移无显著性相关 (P >0 .0 5 )。　结论 :GABA和Glu参与胃癌的发生 ,有望成为胃癌诊断、治疗的新方向。Objective: To evaluate the contents of γ aminobutyric acid(GABA) and glutamate(Glu) in gastric cancer tissue and normal tissue surrounding the cancer. Methods: 30 patients of gastric cancer underwent surgical treatment were examined. A high performance liquid chromatographic method(HPLC) was applied for the analysis and quantification of GABA and Glu in gastric cancer tissues and normal tissues surrounding the lesion after precolumn derivatization with phenylisothyocyanate (PITC). GABA and Glu were determined with UV detector. The differences in GABA and Glu contents in gastric cancer tissues were regarded to have close link of lymph node metastasis and histological type. Results: GABA and Glu contents were high significantly in gastric cancer tissue comparing with those of surrounding normal tissue( P <0.05). GABA and Glu levels in gastric cancer tissue were not significantly correlated with lymph node metastasis and histological type ( P >0.05). Conclusion: GABA and Glu are involved in the factors of gastric carcinogenesis. The results suggest GABA Glu system detection should be included into the diagnosis and therapy consideration in gastric cancer.

关 键 词：r-氨酪酸 谷氨酸 高效液相色谱法 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]