不同价态锰对神经母细胞瘤细胞的氧化损伤作用  

Lipid Peroxidation Actions of Manganese of Different Valence in SH-SY5Y Cells

作　　者：宣登峰[1] 李洁[2] 张杰[1] 吴萍[1] 褚金花[1] 李国君[1] 

机构地区：[1]首都医科大学,北京100054 [2]北京市丰台区疾病预防控制中心,北京100071

出　　处：《工业卫生与职业病》2004年第4期219-222,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金 ( 3 0 0 0 0 14 0 ) ;北京市科技新星计划资助 ( 95 5 810 2 80 0 )

摘　　要：目的　探讨不同价态、不同浓度锰引起神经细胞的脂质过氧化对细胞的损伤作用。方法　分别用 0 ,0 2 5 ,0 5 0 ,0 75mmol/L二价锰、三价锰对神经母细胞瘤细胞 (SH SY5Y)染毒 2 4h ,观察测定细胞超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)、丙二醛 (MDA)、GSH Px/MDA及细胞膜脂荧光各向异性的变化。结果　二价锰、三价锰染毒各低、中、高剂量组细胞SOD活力 [(5 2 2 1 2± 5 7 74 ) ,(4 98 38± 37 2 9) ,(4 6 4 86± 2 4 0 9)和 (4 99 79± 30 86 ) ,(4 77 2 3± 6 1 6 6 ) ,(4 35 95± 74 0 4 )NU/mg蛋白 ]与对照组[(86 1 2 4± 35 74 )NU/mg蛋白 ]比较明显降低 ,差异有显著性 (P <0 0 5 ) ;GSH Px活力 [(1 9 0 4± 5 1 6 ) ,(1 7 74± 5 91 ) ,(1 5 99± 5 6 2 )和 (1 6 5 8± 6 1 9) ,(1 5 79± 7 2 9) ,(1 4 2 3± 5 2 0 )活力单位 ]均较对照组[(2 9 2 3± 7 83)活力单位 ]降低 ,差异有非常显著性 (P <0 0 1 ) ;GSH Px/MDA较对照组降低 ,差异有显著性 (P <0 0 5 ) ,而MDA和细胞膜脂荧光各向异性有所升高 (P <0 0 5 ) ;相同染毒剂量情况下 ,三价锰组上述指标的变化比二价锰组明显。结论　锰通过抑制SOD、GSH Px活力 ,降低细胞抗氧化能力 ,造成细胞膜功能的损伤。三?Objective To study the level and action of lipid peroxidation induced by manganese of different valence and dosage in SH SY5Y cells.Methods The SH SY5Y cells were incubated in culture with 0 25,0 50 and 0 75 mmol/L manganese for 24 hours respectively.The activities of SOD and GSH Px,the level of MDA,the ratio of GSH Px/MDA and the membrane fluidity were detected.Results Results showed that activities of SOD treated by Mn 2+ and Mn 3+ in low,media and high level respectively[(522 12±57 74),(498 38±37 29),(464 86±24 09),(499 79±30 86),(477 23±61 66),(435 95±74 04) NU/mg protein] were significantly lower than those in untreated cells(P<0 01).The activities of GSH Px[(19 04±5 16),(17 74±5 91),(15 99±5 62),(16 58±6 19),(15 79±7 29),(14 23±5 20)U] were lower than those in untreated cells(P<0 01), and GSH Px/MDA of the cell treated by Mn 3+ and Mn 2+ were lower than those of the untreated cells,while the MDA and the difference of the membrane fluidity increased greatly as compared to controls(P<0 05).With the treatment in the same dose,the change in cells treated with Mn 3+ was more significant than that in cells treated with Mn 2+ .Conclusions Manganese could promote lipid peroxidation,and cause membrane fluidity to decrease.The toxicity effects of Mn 3+ are more powerful than that of Mn 2+ .

关 键 词：锰 脂质过氧化 神经毒性 神经母细胞瘤细胞 

分 类 号：R114[医药卫生—卫生毒理学]