BCG-DNA对哮喘患者外周血单个核细胞IFN-γ和IL-4产生的作用  

Effect of BCG DNA on IFN- γand IL-4 secreted by peripheral blood mononuclear cells in asthma patients

作　　者：商艳[1] 胡振林[2] 李强[1] 施柯[2] 刘忠令[1] 孙树汉[2] 

机构地区：[1]第二军医大学长海医院呼吸内科,上海200433 [2]第二军医大学基础医学部医学遗传学教研室

出　　处：《第二军医大学学报》2004年第7期718-720,共3页Academic Journal of Second Military Medical University

基　　金：国家高新技术发展规划 (" 863"计划 )课题( 2 0 0 1AA2 13 111)

摘　　要：目的 :研究免疫活性物质卡介苗基因组 DNA(BCG- DNA)对哮喘患者外周血单个核细胞 (PBMC) IFN- γ和 IL- 4产生的影响。 方法 :分离哮喘患者 PBMC,分别加入 BCG- DNA、地塞米松刺激培养 ,EL ISA检测培养上清中 IFN- γ及 IL- 4含量 ,RT- PCR检测 PBMC IFN-γ m RNA及 IL - 4 m RNA的表达量 ,并与空白对照组相比较。结果 :地塞米松在体外对哮喘患者PBMC产生的 IFN-γ m RNA和蛋白、IL - 4 m RNA和蛋白均有抑制作用 ;BCG- DNA抑制哮喘患者 PBMC IL - 4 m RNA和蛋白的分泌 ,而促进 IFN -γ m RNA和蛋白的分泌 ,与对照组比较差异显著 (P<0 .0 1 ) ,且在体外对 PBMC无毒性。 结论 :BCG-DNA不仅具有和地塞米松相同的下调 IL - 4表达的作用 ,而且还具有地塞米松所没有的诱导 IFN -γ表达的作用 ,利于纠正哮喘患者体内存在的 Th1 / Th2比例失衡状态 。Objective:To investigate the effect of BCG-DNA on IFN-γand IL-4secreted by the peripheral blood mononuclear cells(PBMC)in asthma patients.Methods:BCG-DNA and dexamethasone were added into PBMCs respectively. IFN-γand IL-4in the supernatant were detected by ELISA;mRNA level was evaluated by reverse transcript polymerase chain reaction.The results were compared among BCG-DNA,dexamethasone and control groups.Results:Dexamethasone in vitro inhibited the expression of Th1cytokine IFN-γand Th2cytokine IL-4in PBMC BCG-DNA inhibited IL-4expression in PBMC and promoted IFN-γexpression,and compared to control group there was significant difference(P<0.01).BCG-DNA had no toxicity to PBMC.Conclusion:BCG-DNA can down-regulate IL-4and induce IFN-γsecretion,which may help to re-verse Th1/Th2imbalance in asthma patients for therapeutic purpose.

关 键 词：BCG-DNA 哮喘 外周血 单个核细胞 IFN-R IL-4 

分 类 号：R562.25[医药卫生—呼吸系统]