蛋白激酶C的活性变化对人肝癌细胞增殖及端粒酶表达的影响  

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

作　　者：张海伟[1] 罗英儒[2] 郑佩娥[1] 文剑明[3] 

机构地区：[1]暨南大学医学院病理解剖学教研室,广东广州510080 [2]暨南大学医学院药理学教研室,广东广州510632 [3]中山大学基础医学院病理解剖学教研室,广东广州510080

出　　处：《中国病理生理杂志》2004年第7期1176-1178,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .395 0 0 0 5 6 ) ;广东省"五个一兴教工程"基金资助项目

摘　　要：目的 :揭示蛋白激酶C(PKC)活性变化对人肝癌细胞增殖及端粒酶表达的影响。方法 :体外以PKC激活剂PMA(phorbol- 12 -myristate - 13-acetate)及其抑制剂星形孢菌素 (staurosporine,SP)作用于人肝癌细胞系 (BEL- 74 0 2 ) 4 8h后 ,运用TRAP -银染 (telomericrepeatamplificationprotocolsilverstaining)与计算机图像凝胶扫描记录技术结合的方法来揭示细胞端粒酶活性、PKC变化与细胞增殖的关系。结果 :PMA与SP的作用 ,促进其细胞增殖抑制的同时 ,抑制其细胞端粒酶活性表达。结论 :PKC的活性变化与人肝癌细胞增殖相关 。AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC. [

关 键 词：肝肿瘤 增殖 蛋白激酶C 端粒 末端转移酶 

分 类 号：R735.7[医药卫生—肿瘤]