灭活SARS冠状病毒疫苗对小鼠脏器影响的实验研究  

Effect of inactivated SARS coronavirus vaccine on mouse organs

作　　者：杜彬[1] 钟雪云[1] 熊盛[2] 张传海[2] 刘新建[2] 刘石生[2] 张美英[2] 李久香[2] 王一飞[2] 陆家海[3] 万卓越[4] 鄢心革[4] 郑焕英[4] 范江林[5] 

机构地区：[1]暨南大学医学院病理解剖教研室,广东广州510632 [2]暨南大学生物医药研究开发基地,广东广州510632 [3]中山大学公共卫生学院,广东广州510089 [4]广东省疾病预防控制中心,广东广州510300 [5]日本筑波大学

出　　处：《中国病理生理杂志》2004年第7期1187-1189,共3页Chinese Journal of Pathophysiology

基　　金：广东省非典科技攻关专项 (No .2 0 0 32 3-E -4 6 1) ;教育部科技研究重点项目 (No .10 4 181) ;广东省自然科学基金重点项目 (No . 0 132 0 3) ;广州市科技攻关项目(No .2 0 0 3Z3-E0 4 0 1)

摘　　要：目的 :探讨灭活SARS冠状病毒疫苗对小鼠是否有损伤作用。方法 :用灭活SARS冠状病毒分别免疫BALB/c和C5 7BL/ 6小鼠 ;ELISA法检测小鼠血清中抗SARS冠状病毒抗体水平 ;免疫 8周后取小鼠各脏器 ,观察其病理形态学改变。结果 :免疫 8d后 ,小鼠血清中就可检测到特异的IgM、IgG抗体 ;组织切片染色观察 ,可见少数小鼠肺及肝组织出现轻度损伤 ,其他脏器未见病变。结论 :SARS冠状病毒灭活疫苗可诱导小鼠产生特异性抗体 ,同时没有造成严重的器官损伤。AIM: To study the pathological change in mouse organs immunitied by inactivated SARS-CoV vaccine. METHODS: Inactivated SARS-CoV vaccine was injected into BALB/c and C57BL/6 mice. Anti-SARS antibody was analyzed by ELISA. After 8 weeks, the immunitied mice were killed and those organs were analyzed by pathological methods. RESULTS: Anti-SARS antibody in mice was positive after 8 days. Only minimal injury was observed in a few lungs and livers, but the other organs were not. CONCLUSIONS: Inactivated SARS-CoV vaccine induced mice to create antibody, whereas they did not cause severe injury. This result will be valuable for vaccine into clinical research. [

关 键 词：严重急性呼吸综合征 冠状病毒 疫苗 灭活 小鼠 

分 类 号：R363[医药卫生—病理学]