内皮素受体在雄激素非依赖性前列腺癌中的作用  

The effect of endothelin receptor in androgen-independent prostate cancer

作　　者：薄隽杰[1] 黄旭元[1] 孙杰[1] 戴胜国[1] 王益鑫[1] 

机构地区：[1]上海第二医科大学附属仁济医院泌尿外科,200001

出　　处：《中华外科杂志》2004年第14期870-873,共4页Chinese Journal of Surgery

摘　　要：目的　研究雄激素非依赖性前列腺癌株PC3中内皮素 (ET)受体表达及受体阻断后PC3细胞的凋亡情况。方法　RT PCR法测定PC3中ET受体的表达以及采用受体拮抗剂干预后其表达的变化 ,通过流式细胞仪和透射电镜研究干预后PC3细胞凋亡的情况。结果　PC3中ETA表达较高 ,而ETB表达非常弱。ETA受体拮抗剂干预后 ,ETA表达明显减少 ,随干预浓度的增加 ,PC3细胞凋亡的比例逐渐增加 ;而ETB受体拮抗剂干预后无明显改变。结论　PC3中ET 1的作用主要通过ETA受体介导 ,ETB受体是静止的。ETA受体阻断后 ,表达减少 ,PC3细胞出现凋亡 。Objective To study the expression of ET receptor and the apoptosis after intervened with ET receptor antagonist in androgen independent prostate cancer Methods PC3,an androgen independent prostate cancer cell line,was used The expression of ETA and ETB receptor in PC3 was measured through RT PCR After intervened with selective ETA and ETB receptor antagonist,the apoptosis in PC3 was studied through flow cytometry and electron microscope Results Clear signal was obtained in PC3 for ETA receptor mRNA transcript,while the signal for ETB receptor mRNA transcript was very weak The expression of ETA receptor mRNA was obviously reduced and the apoptosis of PC3 cell was observed after intervened with selective ETA receptor antagonist There was no change after intervened with selective ETB receptor antagonist Conclusion ET 1 exerts its effects through the ETA receptor subtype and ETB receptor is silenced in PC3 The expression of ETA was reduced and the apoptosis was observed in PC3 when ETA receptor was blocked It was dose dependent

关 键 词：内皮素受体 雄激素非依赖性前列腺癌 PC3细胞 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]