层-层自组装构建固相可降解基因传递体系的研究  

Construction of Biodegradable Multilayer Films via Layer-by-layer Self-assembly as Gene Delivery System

作　　者：任科峰[1] 计剑[1] 沈家骢[1] 

机构地区：[1]浙江大学高分子科学与工程学系,杭州310027

出　　处：《高等学校化学学报》2004年第8期1576-1578,M009,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :5 0 3 73 0 3 6)资助

摘　　要：Biodegradable multilayer films of alternating poly- L -lysine(PLL) and deoxyribonucleic acid(DNA) layers were fabricated onto quartz substrates by the layer-by-layer(LbL) self-assembly method. UV-Visible spectra showed that PLL and DNA can successfully be adsorbed and form multilayer films via LbL. Degradation experiments were carried out with enzymes. AFM was utilized to measure the changes of thickness of multilayer films. The gradual decrease of thickness of multilayer films with the increase of degradation time was observed. In our experiments, about 85% of films was degraded in 35 h. Our study provided a novel gene delivery system using LbL method,which may be applied to gene therapy.Biodegradable multilayer films of alternating poly- L -lysine(PLL) and deoxyribonucleic acid(DNA) layers were fabricated onto quartz substrates by the layer-by-layer(LbL) self-assembly method. UV-Visible spectra showed that PLL and DNA can successfully be adsorbed and form multilayer films via LbL. Degradation experiments were carried out with enzymes. AFM was utilized to measure the changes of thickness of multilayer films. The gradual decrease of thickness of multilayer films with the increase of degradation time was observed. In our experiments, about 85% of films was degraded in 35 h. Our study provided a novel gene delivery system using LbL method,which may be applied to gene therapy.

关 键 词：生物降解 聚赖氨酸 基因传递 层-层自组装 

分 类 号：O631.3[理学—高分子化学]