混合稀土常乐对孕鼠胚胎细胞的DNA损伤作用  

Effects of Mixed Rare Earth Changle on DNA Damage of Embryo Cell of Pregnancy Rat

作　　者：周莉[1] 李树蕾[1] 陈辉[1] 黄可欣[1] 聂毓秀[1] 

机构地区：[1]吉林大学基础医学院组织胚胎学教研室,吉林长春130021

出　　处：《中国稀土学报》2004年第3期390-392,共3页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金资助项目 ( 2 9890 2 80 3 )

摘　　要：为评估农用稀土常乐对人体胚胎的影响 ,采用微核试验和单细胞凝胶电泳技术检测混合稀土常乐能否通过胎盘屏障造成胎儿细胞DNA损伤。于雌鼠妊娠第六天开始染毒 ,每天分别以 0 .3 ,2 ,5和 2 0mg·kg- 1 混合稀土常乐灌胃 ,直至妊娠第 18d。结果显示 :除 0 3mg·kg- 1 剂量组外 ,其他各剂量组微核细胞率显著高于对照组 ,呈剂量—反应关系。单细胞凝胶电泳结果显示 ,随着染毒剂量的增加 ,彗星细胞率增加 ,也呈剂量—反应关系 ,由此得出结论 :农用稀土常乐在 2 ,5和 2 0mg·kg- 1 剂量下可不同程度地通过胎盘屏障 ,并引起胚胎肝细胞和多染红细胞DNA损伤。To assess the potential risk of mixed rare earths Changle for human embryo transplacental micronucleus test and single cell gel electrophoresis (SCGE) technique to detect DNA damage of embryo we used. The rats were administered 0.3, 2, 5 respectively and 20 mg·kg^(-1) mixed rare earths Changle every day orally from the 6th to the 18th day after pregnancy. The results show that the number of cells with micronucleus significantly increases in comparason with the control groups except 0.3 mg·kg^(-1) group, which appeared to be a dose-effect relationship. The number of comet star cell greatly increases with increasing contamination dose in comparason with the control groups except 0.3 mg·kg^(-1) group, and also displayes a dose-effect relationship. In conclusion, though mixed rare earth Changle is restricted by placenta membrane to enter embryo body, more than 2 mg·kg^(-1) mixed rare earth Changle may cross placenta barrier and cause DNA damage of hepatocyte and developing erythrocyte of rat embryo.

关 键 词：细胞生物学 混合稀土 胎盘屏障 胚胎细胞 DNA损伤 稀土 

分 类 号：O614.33[理学—无机化学] R-332.4[理学—化学]