华山松根与针叶凯氏带的比较研究  

Comparative study of Casparian strips in the roots and needles of Pinus armandi

作　　者：唐熙[1] 吴小琴[1] 胡玉熹[1] 林金星[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《西北植物学报》2004年第8期1378-1383,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国科学院创新重点项目基金 KSCXZ-SW-108

摘　　要：应用冰冻切片、酶解分离、荧光显微技术和傅里叶红外光谱分析 FTIR 等手段,对华山松初生根和针叶内皮层凯氏带进行了分离、显微结构特征和化学成分的比较.研究结果表明:针叶凯氏带的 网格 结构比较整齐,大小较一致,排列也较规则,同时在 网格 的纵向壁上具有明显的初生纹孔场.而初生根凯氏带网状结构的大小、排列均不规则,在其 网格 的纵向壁上的初生纹孔场不明显.根据FTIR的检测结果显示:初生根凯氏带中木栓质和木质素的含量均高于针叶,而纤维素的含量则明显低于针叶;两者细胞壁蛋白的含量基本相同.本文的研究结果为深入探讨植物地下部分和地上部分凯氏带的生理功能提供新的佐证.Casparian strips were isolated from primary roots and needles by enzymatic isolation,the cellular structure was observed under the fluorescence microscope and chemical characterization was analyzed by Fourier transform infrared (FTIR) spectroscopy.The results indicate the presence of the primary pit fields in the longitudinal walls of 'net' isolated from the needles,while such structure is not evident in the cell walls of the primary roots.The 'net' from the needles appears to be more orderly,similarly sized,and well aligned;while those from the primary roots are irregular in sizes as well as mesh positioning.The FTIR analysis indicate that the Casparian strips from primary roots contain notably higher amount of suberin and lignin thanthose from the needles,whereas the content of cellulose from primary roots is lower than those from the needles.However,in terms of protein contents in the cell walls,one bears no significant difference from the other.The comparative study provides new reference for the study of physiological functions of Casparian strips in the endodermis of the leaves and roots.

关 键 词：华山松 初生根 针叶 凯氏带 酶解技术 傅里叶变换红外光谱术 

分 类 号：Q944.54[生物学—植物学]