3种芦荟属植物叶的表皮扫描电镜观察及芦荟素含量的测定  

Observation on the epidermis of leaves by scanning electron microscope and the determination of contents of aloin in three species of Aloe

作　　者：李建军[1] 李景原[1] 朱命炜[1] 李俊英[1] 王红星[1] 刘清兰[1] 王太霞[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002

出　　处：《西北植物学报》2004年第8期1397-1401,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：以库拉索芦荟、木立芦荟和皂质芦荟为材料,用扫描电镜观察其叶表皮气孔和角质膜的结构;用高效液相色谱法 HPLC 测定了3种芦荟属植物叶中芦荟素的含量.扫描电镜观察结果表明,3种芦荟叶表皮都覆盖有厚的角质膜,气孔下陷,表现出典型的旱生植物特征.但角质膜的纹饰和厚度在不同芦荟间有显著差异.木立芦荟角质膜表面呈瘤状突起,角质膜厚度为5～6μm;库拉索芦荟和皂质芦荟的角质膜表面较平,库拉索芦荟的角质膜厚度为3～4μm,皂质芦荟的角质膜厚度为8～10μm.高效液相色谱法 HPLC 测定结果表明,木立芦荟叶含芦荟素最高,库拉索芦荟叶含量较低,而皂质芦荟叶含量最低.此外,本文还初步探讨了芦荟属植物叶表皮结构与芦荟素含量的关系.The leaves of three species (Aloe vera,A.arborescens,A.saponaria) of Aloe were used as material.The structure of stoma and cuticle of the epidermis were observed by scanning electron microscope and the contents of aloin were determined by HPLC.The results showed that all the epidermises of the leaves were covered with heavy cuticle and sunken stomata,which were the typical characteristics of xerophytes.However the decorative pattern and the thickness of cuticle varied differently among the three species.The cuticle surface was in shape of tumour in Aloe arborescens,comparatively,the cuticle surface was smoother in Aloe vera and A.saponaria.The thickness of cuticle in Aloe arborescens was 5~6 μm,3~4 μm in Aloe vera,and 8~10 μm in Aloe saponaria.The order of the three species in the contents of aloin was that Aloe arborescens,Aloe vera and A.saponaria.The relationship between the structure of epidermis and content of aloin was also discussed in this paper.

关 键 词：芦荟属 角质膜 扫描电镜 芦荟素 

分 类 号：Q944.56[生物学—植物学]