宋氏Ⅰ相抗原质粒的分子克隆及与Ⅰ相抗原突变株的互补检测  

Molecular Cloning of Form Ⅰ Plasmid and Complementary Test with Form Ⅰ-negative Mutants

作　　者：林应锵[1] 詹丽钦[1] 温彩霞[1] 包幼迪[1] 

机构地区：[1]福建医学院基因工程研究室

出　　处：《福建医学院学报》1993年第1期1-4,共4页

基　　金：国家自然科学基金

摘　　要：用“鸟枪”法克隆宋氏痢疾杆菌Ⅰ相抗原质粒 pFEG 001,获得13株重组子。将其分别导入宋氏痢疾杆菌Ⅰ相抗原突变株 BW 201～204进行互补检测,发现重组质粒 pBL 108可反式互补于Ⅰ相抗原突变质粒 paW 204,恢复Ⅰ相抗原的表达;该重组质粒中的插入片段分子量为1.0kb。互补结果提示该片段内至少含有1个与Ⅰ相抗原表达有关的基因,据此推测宋氏Ⅰ相抗原的表达受多基因控制。Bam HI-generated DNA fragments of pFEG 001 were cloned into E.coli C600 with plasmid vector pBR 322 by shot-gun cloning,and 13 recombinants were obtained.All were Form Ⅰ-negative.Each of recombinant plasmids was introduced into the Tn5-insertion mutant BW 201～207 cells by electropora- tion.The results of eomplementation test indicated that the recombinant pBL 108 containing the 1.0 kb Bam HI DNA fragment of pFEG 001 was able to restore the synthesis of Form Ⅰ antigen in BW 204 cells harbouring the Tn5-insertion mutant plasmid.Thus,the 1.0 kb Bam HI DNA sequence contains at least one gene related to Form Ⅰ antigen expression.

关 键 词：宋氏 痢疾杆菌 I相抗原 分子克隆 

分 类 号：R394.8[医药卫生—医学遗传学]