幽门螺杆菌vacA基因多态性与慢性胃病的关系  

Relation between diversity of vacA of Helicobacter pylori and chronic gastric diseases

作　　者：叶桂安[1] 张万岱[1] 刘利民[1] 施理[1] 徐智民[1] 陈烨[1] 周殿元[1] 

机构地区：[1]第一军医大学南方医院消化内科研究所,广东省广州市510515

出　　处：《现代消化及介入诊疗》2000年第2期32-35,共4页Modern Interventional Diagnosis and Treatment in Gastroenterology

摘　　要：目的探讨幽门螺杆菌(Hp)vacA 基因多态性与慢性胃病的关系。方法经胃镜检查活检胃粘膜标本,分离鉴定 Hp,酚氯仿方法抽提 DNA,聚合酶链反应检测 vacA 基因的信号区(s)和中间区(m)。结果 92例 Hp 临床分离株中,sla/ml 型14株(15.2%)、sla/mla 型6株(6.5%)、sla/m^2型55株(59.8%)、slb/ml 型2株(2.2%)、sla/m2型5株(5.4%)、s2/m2型8株(8.7%),另有2株 sla 型 Hp 不通按 ml、mla 或 m2进一步分型,未发现 s2/ml、slb/mla 及 S2/mla 型。除4例胃癌外,其余88例按消化性溃疡和慢性胃炎分组,40例消化性溃疡患者感染 Hp 中 sla 型37株,占92.5%,slb 型2株(5%),s2型1株(2.5%);ml 型4株(10%),mla 型2株(5%),m2型32株(80%),另有2株 sla 型 Hp 未能按 ml、mla 或 m2进一步分型。48例慢性胃炎患者感染Hp 中 sla 型36株,占75%,slb 型5株(10.4%),s2型7株(14.6%);ml 型11株(22.9%),mla 型4株(8.3%),m2型33株(68.8%)。消化性溃疡组慢性胃炎组均以 sla 型、m2型为主,分别比较两组间 sla 型及 m2型的分布,两组间 sla 型有显著性差异(P<0.05),而 m2型无显著性差异(P>0.05)。结论慢性胃病患者感染 Hp 主要为 sla/m2型,其次为 sla/ml 型。sla 型 Hp 与消化性溃疡的发生密切相关。AIM To analysis relation between the diversity of vacA of Helicobacter pylori and chronic gastric diseases. METHODS Gastric biopsy specimens for culture were taken by using a sterilized endoscope,92 strains H.pylori clincal isolates from patients were cultured and collected,DNA was extracted by the phenol-chlo- roform method,vacA signal sequences and middle-region alleles were amplified by the method of poly- merase chain reaction (PCR). RESULTS 92 clinical isolates of H.pylori were characterized by vacA typing.14(15.2%) of 92 isolates were sla/ml,6(6.5%) were sla/mla,55(59.8%) were sla/m2,2(2.2%) were slb/ml,5(5.4%) were slb/ m2,8(8.7%) were s2/m2,only 2 could not be typed,s2/ml or slb/mla or s2/mla isolates had not been found.37(92.5%) of 40 isolates from patients with peptic ulceration were sla,2(5%) were slb,1 (2.5%) was s2;4(10%) were ml,2(5%) were mla,32(80%) were m2,the midregions from 2 isolates from patients with peptic ulceration could not be typed.36(75%) of 48 isolates from patients with chronic gastritis were sla,5(10.4%) were slb,7(14.6%) were s2;11(22.9%) were ml,4(8.3%) were mla,33(68.8%) were m2.sla and m2 isolates were found more frequently.The form sla between two groups was statistically sig- nificant (p<0.05).but the form m2 between two groups was not different (p>0.05). CONCLUSION These results indicate that the sla/m2-type strains are common in the patients from south-china.The sla forms correlate with peptic ulceration.

关 键 词：幽门螺杆菌 HP vacA基因多态性 慢性胃病 胃镜检查 消化性溃疡 基因亚型 

分 类 号：R573.1[医药卫生—消化系统]