胸腺素a_1的分离纯化  

Isolation and Purification of Thymosin a_1

作　　者：韩香[1] 顾军[1] 苑庆兰[1] 甘一如[1] 

机构地区：[1]中国人民武装警察部队医学院化学教研室,天津300162

出　　处：《中国医药工业杂志》2004年第6期335-337,共3页Chinese Journal of Pharmaceuticals

基　　金：天津市自然科学基金重点资助项目(993804311)

摘　　要：化学合成胸腺素a1采用阴离子交换色谱和制备型RP-HPLC两步纯化。阴离子交换色谱以pH8.0的Tris-HCl为缓冲液,0～0.35mol/L NaCl溶液离子强度梯度洗脱;制备型RP-HPLC采用C18柱,流动相A为0.1%三氟乙酸-H2O,流动相B为0.1%三氟乙酸-90%乙腈,线形梯度10%～35%B,0～60min洗脱,流速2.0ml/min,检测波长214nm。纯化结果采用分析型RP-HPLC和Tricine-SDS-PAGE检测,终产物纯度达98.8%以上。The synthetic thymosin a1 was purified by anion exchange chromatography and preparative RP-HPLC.The anion exchange chromatography was performed with Tris-HCl buffer containing 0～0.35mol/L NaCl by gradient elution.The RP-HPLC detection was carried out with C18 column using gradient elution of buffer A (0.1%TFA-H2O)and buffer B(0.1% TFA-90%CH3CN), the content of buffer B was 10% to 35% in a flow rate of 2.0ml/min within 60min and thedetection wavelength of 214nm. The RP-HPLC and Tricine-SDS-PAGE analysis showed that purity of final product was upto 98.8%.

关 键 词：芴甲氧羰基(Fmoc) 固相多肽合成 胸腺素Α1 纯化:阴离子交换色谱 反相高效液相色 

分 类 号：TQ464.7[化学工程—制药化工] O647.316[理学—物理化学]