酵母人工染色体DNA的大尺度物理图谱分析  

LONG RANGE PHYSICAL MAP OF YAC DNA

作　　者：柴建华[1] 顾杨洪[1] 王乐[1] 张成 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1993年第4期394-398,共5页Journal of Fudan University：Natural Science

摘　　要：以不同PBR322质粒DNA片段作为YAC(yeast artificial chromosome,酵母人工染色体)DNA左右臂探针,使用稀切点限制性内切酶和脉冲电泳(pul-sed field electrophoresis)技术对含有2bA3的800kbYAC作了4种限制酶大尺度物理图谱分析。Different fragments of plasmid pBR322 DNA were used as the left and right arms probes of YAC (Yeast Artificial Chromosome). The rare cutting restriction enzyme and pulsed field electrophoresis were used for long range physical mapping of YACs. A YAC containing 2bA3 DNA locus with the length of 800 kb was analysed with four different restriction enzyme to obtain the long range physical map.

关 键 词：YAC DNA 酵母 大尺度 物理图谱 

分 类 号：Q343.2[生物学—遗传学]