宁神灵冲剂和脱氢表雄酮对慢性轻度应激小鼠认知功能的影响  

Effect of Ningshen Ling and dehydroepiandrosterone on the cognitive function in mice subjected to chronic mild stress

作　　者：杨楠[1] 高瑞丰 左萍萍[1] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院药理系,北京市100005 [2]希波制药有限责任公司,山东威海市264200

出　　处：《中国康复理论与实践》2004年第5期268-270,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家重点基础研究发展计划基金项目 (No .973 .G2 0 0 0 0 570 0 8)

摘　　要：目的观察慢性轻度应激 (CMS)小鼠模型的自发与认知行为变化 ,中枢胆碱能毒蕈碱型 (M )受体的改变及中药宁神灵冲剂和脱氢表雄酮 (DHEA)的干预作用。方法连续 3周对小鼠采用 7种不可预知的应激原建立CMS模型 ,于应激 2周后进行药物治疗 1周。用自发运动和水迷宫评定行为改变 ,用放射性配基法测定皮层、海马、下丘脑和小脑的M受体改变。结果未治疗的CMS小鼠自发运动明显减少 ,寻找平台潜伏期明显延长 (P <0 0 1)。经服用宁神灵冲剂和DHEA后可有效改善上述行为变化。CMS小鼠大脑皮层、海马的M受体特异性结合与对照组比较减少 (P <0 0 5 ) ,经上述两种药物治疗后恢复至正常水平。结论CMS可使动物的自发运动减少 ,并使其空间学习记忆功能减退 ,该变化与脑内胆碱能受体活性低下有关。中药宁神灵治疗后与DHEA一样可有效改善上述变化。ObjectiveTo investigate the alteration of behavior,such as spontaneous movement,spatial memory ability and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS). And to determine whether Ningshen Ling (NSL)and dehydroepiandrosterone(DHEA)could reverse the cognitive impairment in this model.MethodsForty mice were divided into four groups: control group,CMS group,CMS+NSL(p.o. 5 g·kg -1 ·d -1 ) group and CMS+DHEA (i.p. 5 mg·kg -1 · d -1 ) group. Morris water maze procedure was used to determine the spatial memory ability. M receptor binding activity was measured with radioactivity assay by 3H-QNB.ResultsAfter 3 weeks CMS,the mice exerted a decrease in spontaneous movement test,and the latency in Morris water maze was obviously prolonged. Treating CMS mice with NSL and DHEA for 1 weeks could improve the spontaneous movement and latency declined. Whereas the 3H-QNB binding ability to M receptor showed a significantly decrease in cerebral cortex and hippocampus of CMS mice ( P <0.05),the decreased ability of M receptor binding was reversed by NSL treatment in hippocampus( P <0.05).ConclusionNSL and DHEA can alleviate the stress response and reversed cognitive impairment induced by CMS,and it may be concerned with the central cholinergic M receptors activity.

关 键 词：慢性轻度应激(CMS) 宁神灵 脱氢表雄酮(DHEA) M胆碱能受体 自发运动 Morris水迷宫 小鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]