视网膜脱离大鼠视网膜核增殖抗原表达与白细胞介素-1β的关系  

Level of proliferating cell nuclear antigen and interleukin-1β in rats' experimental retinal detachment

作　　者：卢华[1] 马志中[2] 刘敬[1] 曹利群[1] 

机构地区：[1]中国人民解放军总医院,北京市100853 [2]北京大学第三医院眼科中心,北京市100083

出　　处：《中国康复理论与实践》2004年第5期276-278,F003,共4页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探讨白细胞介素 1β(IL 1β)在视网膜脱离状态下与视网膜神经上皮层增殖的关系。 方法用SD大鼠制作大鼠视网膜脱离模型及脱离后不同时间复位模型。流式细胞仪检测各大鼠核增殖抗原 (PCNA)在视网膜神经上皮层的表达。免疫组织化学法定位IL 1β的表达位置。放射免疫法检测IL 1β浓度在视网膜神经上皮层中的改变。应用IL 1β抗体 10 0 0ng、IL 1Ra 2 0ng在PCNA表达的最高点之前视网膜下腔注射 ,与注射等量 0 0 1MPBS对照组进行比较。结果IL 1β在视网膜脱离眼表达于视网膜神经上皮的Muller细胞、星形胶质细胞、血管内皮细胞的胞浆。IL 1β表达的高峰出现在视网膜脱离后第 7天 ,然后维持在较低的表达状态。PCNA在脱离后的第 2天开始表达 ,在脱离后的第 10天达到高峰 ,然后下降 ,并维持在较低的水平。IL 1β抗体、IL 1Ra可以抑制PCNA的表达。结论炎性因子IL 1β与脱离时神经网膜的增殖相关。ObjectiveTo investigate the possible effect of interleukin-1β(IL-1β)in experimental retinal detachment,and the role in proliferation.MethodsThe experimental retinal detachment and reattachment in different time were made using Sprague-Dawley rats. The expression of proliferating cell nuclear antigen (PCNA) in neuro-retina was tested with flow cytometry at different time. IL-1β in neuro-retina were analyzed by labeling with polyclonal IL-1β antibody. The level of IL-1β in neuro-retina were tested with radio-immune method. IL-1β antibody 1000 ng,IL-1Ra 20 ng were injected in the subretinal space of some rats before PCNA reached to its high point respectively,and the expression of PCNA of them were compared with that of control which were injected with 0.01 M PBS.ResultsIL-1β expressed in Muller cell,astrocyte,vascular endothelial cell in neuro-retina and reached to its peak at the 7th day after detachment,then declined and continued at a low level as long as the retina detached.The expression of PCNA began at the second day after detachment, and reached a maximum at about 10 days after,then declined and continued at a low level as long as the retina detached. IL-1βantibody and IL-1Ra could restrain the expression of PCNA.ConclusionProinflammatory cytokine IL-1β is the key factor in proliferation of experimental retinal detachment.

关 键 词：白介素-1Β 核增殖抗原 视网膜脱离 增殖性视网膜病变 大鼠 

分 类 号：R774.1[医药卫生—眼科]