变形链球菌耐氟菌株、远缘链球菌耐氟菌株与亲代菌株基因组AP-PCR的比较  

The genomic differences between the fluoride-resistant strains of Streptococcus mutans and Streptococcus sobrinus and their parental strains by AP-PCR

作　　者：朱怡玲[1] 吴补领[1] 孟艳玲[1] 苏凌云[1] 王敬[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2004年第7期382-385,共4页Chinese Journal of Conservative Dentistry

基　　金：全军十五重点课题 ( 0 1Z0 89);全国高等学校骨干教师资助计划

摘　　要：目的 :利用随机引物聚合酶链反应 ( AP-PCR)技术 ,对变形链球菌、远缘链球菌和耐氟菌株的基因组进行比较 ,判定变形链球菌耐氟突变、远缘链球菌耐氟突变后 ,遗传型是否发生改变。方法 :采用 AP-PCR技术 ,经多次实验自行确立 AP-PCR反应条件 ,对变形链球菌、远缘链球菌及耐氟菌株的基因组进行比较。结果 :变形链球菌耐氟菌株、远缘链球菌耐氟菌株的基因组已发生改变。结论 :变形链球菌耐氟突变、远缘链球菌耐氟突变后 ,已具有自身的遗传特性。AIM:To investigate the genomic differences between the fluoride-resistant strains of Streptococcus mutants and Streptococcus sobrinus and their parental strains.METHODS:We established the right Arbitrarily Primed Polymerase Chain Reaction(AP-PCR) reaction model for the strains.We compared the genomic differences between fluoride-resistant strains and their parental strains by the AP-PCR.RESULTS:The genomes of fluoride-resistant strains were much different from their parental strains.CONCLUSION:Compare to the genomes of their parental strains,the genomes of the fluoride-resistant strains are significantly mutated. They have their own characters.

关 键 词：变形链球菌 远缘链球菌 耐氟菌侏 

分 类 号：R780.2[医药卫生—口腔医学]