阿魏酸钠对肝星状细胞中I型前胶原和CTGF mRNA表达的影响  

Effects of Sodium Ferulate on Type I Procollagen and Connective Tissue Growth Factor mRNA Expression in Rat Hepatic Stellate Cells

作　　者：黄瑾[1] 胡晋红[1] 蔡臻[1] 张万国[1] 

机构地区：[1]中国人民解放军第二军医大学长海医院药学部,上海200433

出　　处：《解放军药学学报》2004年第3期164-166,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　考察阿魏酸钠对氧化应激条件下 ,肝星状细胞中I型胶原及其相关调控因子CTGFmRNA表达的干预作用。方法　以 5 0 μmol·L-1H2 O2 处理的HSC -T6细胞为氧化应激模型组 ,采用RT -PCR方法检测并分析 12 5 μmol·L-1和 5 0 0 μmol·L-1SF对细胞中I型前胶原及其相关调控因子CTGF表达的影响。结果　肝星状细胞在氧化应激条件下I型前胶原mRNA和CT GFmRNA表达水平都明显上调 ,而阿魏酸钠可以下调二者的表达水平。Aim To study the effects of Sodium Ferulate(SF) on type I procollagen and connective tissue growth factor(CTGF) mRNA expression in rat hepatic stellate cells (HSC-T6) stimulated with hydrogen peroxide(H 2O 2).Methods Cultured HSC-T6 cells treated with H 2O 2 served as control group,the effects of SF on type I procollagen and CTGF mRNA level were determined by reverse transcription polymerase chain reaction(RT-PCR).Results On the oxidative stress conditon,type I procollagen and CTGF mRNA level were remarkably increased compared with untreated group.However,the expression of both of them can be down-regulated by SF.Conclusion SF may potential against hepatic fibrosis by regulating the exprssion of collagen at a pretranslational level.

关 键 词：阿魏酸钠 肝星状细胞 Ⅰ型前胶原 CTGF MRNA表达 结缔组织生长因子 氧化应激 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]