葡萄籽多酚对人乳腺癌细胞MCF-7/ADR在裸鼠体内的多药耐药逆转作用  

Reversal of multidrug resistance of MCF-7/ADR in nude mice by grape seed polyphenol

作　　者：张翠娟[1] 周庚寅[1] 李丽[1] 马丽丽[1] 高鹏[1] 李红[1] 

机构地区：[1]山东大学医学院病理教研室,济南250012

出　　处：《中华外科杂志》2004年第13期795-798,共4页Chinese Journal of Surgery

基　　金：山东省卫生厅 95攻关项目(省卫 95攻关 0 2号 )

摘　　要：目的　研究葡萄籽多酚 (GSP)对人乳腺癌耐阿霉素细胞MCF 7/ADR的体内多药耐药逆转作用。方法　采用人乳腺癌裸鼠移植瘤模型研究GSP对肿瘤多药耐药的体内逆转作用 ;采用流式细胞术测定不同用药P 糖蛋白 (Pgp)表达变化及肿瘤细胞凋亡率变化。 结果　体内裸鼠抑瘤实验显示GSP有一定抑瘤作用 ,抑制率为 18 35 % ,联合应用阿霉素 (ADR)可显著抑制肿瘤生长 ,2 0mg/kgGSP可有效逆转MCF 7/ADR细胞的耐药性 ,抑瘤率为 5 4 6 4 %。流式细胞术结果显示应用GSP后肿瘤细胞Pgp表达显著降低 ( 32 0 3± 2 0 9) ,与对照组 ( 5 5 13± 2 12 )相比差异有显著意义 (P <0 0 5 )。平均凋亡率为 15 12 %± 1 0 4 % ,显著高于对照组 9 0 7%± 0 4 3% (P <0 0 5 )。结论　GSP能在体内逆转MCF 7/ADR细胞的耐药性 ,其机制可能是通过抑制Pgp表达 。Objective To study the reversing effect of Grape seed polyphenol (GSP) on multidrug resistance of MCF 7/ADR cell in vivo. Methods The transplantable breast carcinoma cell line MCF 7/ADR model was established in BALB/C nu/nu mice by subcutaneous implantation. Flow cytometry (FCM ) was used to investigate the changes of Pgp expression and apoptosis rate after different drug treatment. Results GSP has some effect on inhibition of tumor growth (the rate of inhibition was 18 35%), and combined with adriamycin can significantly inhibit tumor growth in nude mice, 20 mg/kg GSP can effectively reverse the resistance of MCF 7/ADR cells to ADR in vivo, and the rate of inhibition was 54 64%. FCM results showed that the expression of Pgp was significantly decreased(32 03±2 09)After administration of GSP and ADR, there was distinct difference between it and control(55 13±2 12). The mean rate of apoptosis was 15 12%±1 04%, and was significantly increased compared with control(9 07%±0 43%), P<0 05. Conclusion GSP can effectively reversed the resistance of MCF 7/ADR cells in nude mice, the mechanism may be correlated with inhibition of Pgp expression and apoptosis.

关 键 词：葡萄籽多酚 乳腺癌 癌细胞 MCF-7 ADR 裸鼠 体内 多药耐药逆转作用 

分 类 号：R737.9[医药卫生—肿瘤]