组织工程人工神经诱导管的制备及其在体外与大鼠体内降解性能研究  

Fabrication and biodegradation of poly(DL-lactide-co-glycolide) conduit in rats

作　　者：张文捷[1] 李兵仓[1] 王建民[1] 王建忠[1] 陈建梅[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第六研究室

出　　处：《第三军医大学学报》2004年第13期1165-1168,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 170 96 );国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 6 )~~

摘　　要：目的　以构成比不同的聚乳酸 聚羟基乙酸共聚物 (PLGA)为原料制备人工神经诱导管 ,并对其体内外降解性能变化进行观测。方法　①以 0 2mol LPBS(pH 7 4)为人工降解液 ,观测PLGA ( 85 :15 )和PLGA ( 5 0 :5 0 )管 12周体外降解期间吸水率、失重率及生物降解率变化 ,并以扫描电镜观察其微结构变化 ;②采用肌肉包埋方法 ,观测PLGA ( 85 :15 )和PLGA ( 5 0 :5 0 )管 8周体内生物降解率变化。结果　①体内外降解条件下 ,PLGA ( 5 0 :5 0 )管吸水率、失重率及生物降解率显著高于PLGA ( 85 :15 )管 ;②两种材料样品体内生物降解率显著高于体外降解率 ;③扫描电镜观察显示 ,随降解时间延长 ,材料样品表现为特征性的虫蚀样破坏并逐渐加重 ,以PLGA ( 5 0 :5 0 )管尤为明显。结论　共聚物的构成比以及降解环境是影响聚乳酸 -聚羟基乙酸共聚物生物降解性能的重要因素。Objective To fabricate artificial nerve conduits with two different copolymer proportions of poly (DL-lactide-co-glycolide) (PLGA) [(85:15) or (50:50)] and to investigate the degradation behaviors in vitro and in vivo. Methods Changes in water absorption, weight loss, and degradation of PLGA conduits at the proportions of 85:15 and 50:50 during the 12-week period in pH 7.4 phosphate-buffered saline (PBS) at 37 ℃ and the changes in their in vivo biodegradation following implantation in rat dorsal muscle for up to 8 weeks were observed. Results Compared with PLGA conduit at the proportion of 85:15, significant changes in weight, water absorption, morphology and degradation rate of PLGA conduit at the proportion of 50:50 were found during 12 weeks of incubation with PBS. Both two types of PLGA conduits [(85:15) and (50:50)] showed significantly faster degradation rate in vivo than that in vitro. Conclusion The copolymer proportion and environmental condition have significant effects on the degradation behaviors of PLGA conduits.

关 键 词：聚乳酸-聚羟基乙酸共聚物 生物降解 人工神经诱导管 

分 类 号：R-322[医药卫生] R318.08