卡介菌多糖核酸对肺癌细胞粘附及骨架结构的影响  

Effects of BCG-PSN on the cell adhesion and cytoskeleton structure of lung cancer cells

作　　者：蒋幼凡[1] 沈庆[2] 薛亚梅[1] 

机构地区：[1]重庆医科大学第二临床学院呼吸内科,重庆400010 [2]重庆市第三人民医院老年科,重庆400012

出　　处：《第三军医大学学报》2004年第13期1178-1180,共3页Journal of Third Military Medical University

摘　　要：目的　研究卡介苗多糖核酸 (BCG PSN)对肺癌细胞上粘附分子配体 (slex)表达及肺癌细胞质内骨架的表现特征的影响。方法　采用流式细胞仪及透射电镜观察BCG PSN对高转移人肺巨细胞癌 (PG)细胞和低转移人肺腺癌 (PAa)细胞上slex 表达的影响及胞质内骨架结构的变化。结果　①流式细胞仪分析提示PAa细胞slex 表达高 ( 66.8% ) ,PG细胞表达低 ( 5 .72 % )。经BCG PSN处理后 ,PAa细胞slex 表达呈浓度依赖下降。②超微结构观察 :PAa细胞微丝稀疏 ,PG细胞微管微丝减缺。BCG PSN处理后 ,PG细胞微丝较前丰富、完整 ,树样分布 ,PAa细胞仅见少量的微丝。结论　细胞内微管、微丝等细胞骨架成分的改变与癌细胞运动迁徙能力有关 ,BCG PSN对肺癌细胞粘附性的抑制环节不是细胞骨架介导的粘附强化 ,可能是粘附分子表达下降所致的粘附强化启动过程。Objective To study the effects of BCG-PSN on the expression of the receptor of selectins——one of the important cell adhesion molecules, and the characteristics of cytoskeleton structure in lung cancer cells. Methods The effects of BCG-PSN on the expression of sialyl Lewis X (slex) and the cytoskeleton structure of highly metastatic human pulmonary giant cell carcinoma (PG) cells and lowly metastatic human pulmonary adenocarcinoma (PAa) cells were observed by flow cytometry and transmission electron microscopy. Results Flow cytometric results showed that the expression of slex on the surface of PAa cells (66.8%) was higher than that on PG cells (5.72%). After treatment with BCG-PSN, the expression of slex on PAa cells decreased in a dose-dependent manner. Transmission electron microscopy showed that the microtubules and microfilaments were sparse in the PAa cells and diminished in PG cells. After treatment with BCG-PSN, the microfilaments were more abundant than before in PG cells and showed a branch-like appearance, but still remained sparse in PAa cells. Conclusion Changes in the components of the cytoskeleton structure are associated with the ability of the migration and movement of the tumor cells. The inhibitory effect of BCG-PSN on the adhesiveness of lung cancer cells may not be the cytoskeleton-mediated enhancement of adhesion, but the start-up process resulted from the down-regulation of cell adhesion molecules on the surface of lung cancer cells.

关 键 词：卡介菌多糖核酸 肺癌细胞 粘附分子配体 

分 类 号：R730.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]