γ-干扰素诱导大肠癌细胞株肿瘤坏死因子相关凋亡诱导配体及其受体表达的研究  

The inducible TRAIL and its death receptors expression induced by IFN-γ in RKO cell line

作　　者：胡文献[1] 何超[1] 胡晓彤[1] 黄学锋[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院肿瘤外科,杭州310016

出　　处：《中华实验外科杂志》2004年第7期801-803,共3页Chinese Journal of Experimental Surgery

基　　金：浙江省自然科学基金资助项目(302678)

摘　　要：目的探讨IFNγ对肿瘤坏死因子相关凋亡诱导配体(TRAIL)及TRAIL受体(TRAILRs)表达的诱导作用。方法采用半定量逆转录聚合酶链反应(RTPCR)方法检测IFNγ作用大肠癌细胞株(RKO)不同时间后TRAIL及TRAILRsmRNA的表达。采用流式细胞术检测IFNγ作用不同时间后TRAIL蛋白的表达。结果IFNγ作用后RKO细胞TRAILmRNA的表达量从作用前的0.92上调至1.91,差异有显著性(P<0.05)。细胞表面TRAIL蛋白的表达百分率从作用前的0.64%上升至2.39%。TRAIL死亡受体(DR4、DR5)mRNA的表达量分别从1.32和1.00上调至2.92和2.90,差异均有显著性(P<0.01)。而TRAIL诱骗受体(DcR1、DcR2)mRNA的表达量差异无显著性(P>0.05)。结论IFNγ能够上调TRAIL和TRAIL死亡受体的表达,而对TRAIL诱骗受体无明显影响。Objective To explore the influence of IFN γ on TRAIL and TRAIL Rs in human colon cancer cell line RKO.Methods The expression of mRNA of TRAIL and TRAIL Rs was detected by RT PCR.The expression of TRAIL protein was detected by FACS.Results The expression of TRAIL mRNA were upregulated from 0.92 to 1.91 by IFN γ( P <0.05),and the percentage of TRAIL protein was increased from 0.64% to 2.39%.DR4 and DR5 were upregulated respectively from 1.32 and 1 to 2.92 and 2.90 ( P <0.01),while the changement of TRAIL decoy receptors were not obvious.Conclusion IFN γ can enhance the expression of TRAIL and TRAIL death receptors of RKO,but has no obvious influence on the expression of TRAIL decoy receptors.

关 键 词：Γ-干扰素 诱导 大肠癌 细胞株 肿瘤坏死因子 受体 表达 TRAIL TRAIL-Rs 

分 类 号：R735.34[医药卫生—肿瘤]