改构型和野生型酸性成纤维细胞生长因子对大鼠皮肤生长促进作用的比较  

Effects of reconstructive human acidic fibroblast growth factor and wild type acidic fibroblast growth factor on skin cell proliferation

作　　者：赵京禹[1] 付小兵[1] 陈伟[1] 孙同柱[1] 郑曙云[1] 翁立新[1] 张英民[1] 

机构地区：[1]解放军第三○四医院创伤研究室,北京100037

出　　处：《中国危重病急救医学》2004年第8期458-459,F004,共3页Chinese Critical Care Medicine

基　　金：国家重点基础研究规划 (973 )项目 (G19990 5 42 0 4);国家自然科学基金重点项目(3 0 2 3 0 3 70 );国家自然科学基金面上项目(3 0 170 966)

摘　　要：目的　比较改构型和野生型酸性成纤维细胞生长因子 ( a FGF)促进大鼠皮肤生长的作用。方法　将 2 mm× 2 mm的新生大鼠皮肤分别接种在含有 1、10和 10 0 μg/ L 3种不同浓度的改构型 a FGF和野生型 a FGF与体积分数为 10 %小牛血清的 Dulbecco改良 Eagle培养基中培养 4 d,然后测定生长后皮肤的面积。结果　空白对照组的皮肤面积为 ( 2 .96± 1.12 ) mm2 ,浓度为 1、10和 10 0 μg/ L 改构型 a FGF组的皮肤面积分别是空白对照组的 1.4、1.5和 1.3倍 ,而 3种不同浓度的野生型 a FGF组的皮肤面积分别是空白对照组的 1.5、3.2和 1.6倍。与其他组相比 ,只有浓度为 10μg/ L野生型 a FGF组的皮肤面积显著升高 ( P<0 .0 5 )。结论　改构型 a FGF对皮肤生长无显著刺激 ,其作用效果明显低于野生型 a FGF。Objective To investigate the effects of reconstructive human acidic fibroblast growth factor (aFGF) and wild type aFGF on skin cell proliferation in rat. Methods Neonatal rat skin (area of 2 mm×2 mm ) was cultured in Dulbecco's modification of Eagle's medium containing reconstructive human aFGF and wild type aFGF, respectively. The concentrations of aFGF were 1 μg/L, 10 μg/L, and 100 μg/L. After being cultured for 4 days, the area of skin was measured. Results After treatment with two different growth factors in three different concentrations (1 μg/L, 10 μg/L and 100 μg/L) for 4 days, the areas of skin in three reconstructive human aFGF groups were 1 4, 1 5 and 1 3 fold of that of control, respectively and the areas of three wild type aFGF groups were 1 5, 3 2 and 1 6 fold of that of control, respectively, while the area of skin in the control group was (2 96±1 12) mm 2. In comparison with those of other groups, the skin area of 10 μg/L wild type aFGF group was significantly increased ( P <0 05). Conclusion Reconstructive human aFGF confers less impact on cutaneous cell growth. The capability of wild type aFGF to induce cutaneous cell proliferation is much greater than that of reconstructive human aFGF.

关 键 词：酸性成纤维细胞生长因子 改构型 酸性成纤维细胞生长因子 野生型 皮肤 大鼠 

分 类 号：R363[医药卫生—病理学]