成人急性白血病p16基因的纯合缺失及甲基化研究  

Deletion and Methylation of p16 Gene in an Adult Acute Leukemia

作　　者：宋国英[1] 杨君峥[1] 孙惠[2] 刘长凤[2] 陈辉[1] 李晓雯[1] 

机构地区：[1]郑州大学细胞生物与医学遗传学教研室,河南郑州450052 [2]郑州大学第一附属医院,河南郑州450052

出　　处：《河南肿瘤学杂志》2004年第4期229-231,共3页Henan Journal of Oncology

基　　金：河南省自然科学基金资助项目 9840 2 2 90 0

摘　　要：目的　观察成人急性白血病p16基因的纯合缺失及甲基化的情况。方法　用PCR法对成人急性白血病患者骨髓标本进行p16基因第 1、第 2外显子纯合缺失及甲基化检测。结果　 1) 71例成人急性白血病患者中p16基因纯合缺失 2例 ,均为B ALL ,AML及正常对照组p16基因未见纯合缺失 ;2 ) 2 8例ALL中 ,5例发生异常甲基化 ;43例ANLL中 ,有 7例发生了异常甲基化。结论　p16基因缺失。Objective To discuss the deletion and methylation of p16 gene in adult acute leukemia. Methods Using restrication enzymes PCR techniques. Results 1) Homozygous deletion of p16 gene was only found in 2 patients with ALL, not in patients with AML or in normal samples. 2) In 28 ALL cases, methylation was found in 5 cases and in 43 ANLL cases, methylation was found in 7 cases. Conclusions Deletion and methylation of p16 gene is likely to relate to generation and prognosis of leukemia.

关 键 词：急性白血病 P16基因 甲基化 

分 类 号：R733.2[医药卫生—肿瘤]