大鼠脑缺血再灌注基质金属蛋白酶9表达在脑水肿形成中的作用  

Effect of the expression of matrix metalloproteinase-9 on edema formation in rats after cerebral ischemia-reperfusion

作　　者：魏微[1] 张微微[1] 

机构地区：[1]解放军北京军区总医院神经内科,北京市100700

出　　处：《中国临床康复》2004年第22期4488-4489,i003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39770753)~~

摘　　要：目的:观察大鼠局灶性脑缺血再灌注后基质金属蛋白酶-9(matrixmet-alloproteinase-9,MMP-9)表达的情况,探讨其在缺血再灌注炎性反应和脑水肿形成中的作用。方法:用线栓法建立大鼠局灶性脑缺血再灌注模型,分别观察再灌注后3,6,24,48h,5dMMP-9的表达情况,并测定脑组织含水量。结果:脑缺血再灌注后MMP-9阳性细胞数明显增加,再灌注24h阳性细胞数为(35.81±6.87)个/切片,较再灌注3h组(1.56±1.15)个/切片明显增加(P<0.05),这种增高趋势持续至再灌注后5d。测定脑缺血再灌注后脑含水量,再灌注24d组为(81.49±0.68)%,较假手术组(78.11±1.36)%明显增加(P<0.01)。脑含水量的变化与MMP-9的表达增高趋势一致。结论:MMP-9参与了缺血再灌注血管源性脑水肿、炎症反应等过程并在损伤的修复中可能有重要作用。AIM: To investigate the expression of matrix metalloproteinase-9(MMP-9) in r ats after focal ischemia-reperfusion and to explore its effect on the inflammat ory reaction and the edema formation after ischemia-reperfusion. METHODS:Rat model of focal ischemia-reperfusion was established by middle cer ebral artery occlusion with a nylon monofilament suture.The expressions of MMP- 9 were observed at 3,6,24,48 hours and 5 days respectively after reperfusion and the water content of brain tissue was measured. RESULTS:The number of MMP-9 po sitive cells increased significantly after cerebral ischemia reperfusion.The num ber of positive cells of 24-hour reperfusion group[(35.81±6.87)/slice] was sig nificantly higher than that of 3-hour reperfusion group[(1.56±1.15)/slice](P< 0.05).The increasing tendency lasted to the 5th day after reperfusion. The water content of brain in 24-hour group after reperfusion[(81.49±0.68)%]significan tly increased as compared with that of sham-operation group[(78.11±1.36)%](P< 0.01),and the changes of the water content of brain were in accordance with the increasing tendency of the expression of MMP-9. CONCLUSION:MMP-9 takes part in the process of vasogenic brain edema and the i nflammatory reaction,etc. after ischemia reperfusion, and may play an important role in the rahabilitation of the damage.

关 键 词：脑缺血 再灌注损伤 基质金属蛋白酶-9 

分 类 号：R743[医药卫生—神经病学与精神病学]