水飞蓟宾-磷脂酰胆碱复合物对大鼠脑缺血再灌注损伤的保护作用(英文)  

Protective reactions of Silybin-phosphatidylcholine compound on cerebral ischemic reperfusion injury in rats

作　　者：陈正跃[1,2] 许建文[3] 张光军[3] 

机构地区：[1]华中科技大学同济医学院病理生理学系 [2]新乡医学院药物研究室,河南省新乡市453003 [3]新乡医学院药物研究室

出　　处：《中国临床康复》2004年第22期4629-4631,共3页Chinese Journal of Clinical Rehabilitation

基　　金：河南省科技攻关项目(001180203)~~

摘　　要：frlftflf背景:水飞蓟宾(silybin)具有抗自由基活性、抗脂质过氧化作用和抗脂氧酶作用、极低的毒性,但由于其人体生物利用度低,故影响其疗效。水飞蓟宾-磷脂酰胆碱复合物(Silybin-phosphatidylcholinecompound,SPC)具有较好的生物利用度,并且SPC中所含磷脂酰胆碱也具有抗自由基活性、抗脂质过氧化作用,理论上可以认为SPC具有更好的药理作用。目的:了解水飞蓟宾-磷脂酰胆碱复合物(SPC)对大鼠脑缺血再灌注损伤的保护作用。设计:完全随机设计,对照实验研究。地点和材料:所有研究工作均在新乡医学院药物研究室进行。水飞蓟宾(Silybin,含量98%),辽宁盘锦第三制药厂提供;水飞蓟宾-磷脂酰胆碱复合物(SPC,含量>95%),本室合成;丙二醛(MDA)试剂盒,南京建成生物工程研究所提供。仪器:SHIMADZU2550紫外-可见光分光光度计,武汉科学仪器厂DL-6低温离心机。实验动物有新乡医学院动物中心提供。干预:各组受试大鼠给与供试样品灌胃;灌胃容积为0.5mL/100g。将SPC和Silybin分别悬浮于5g/L羧甲基纤维素钠(CMC)溶液中;①伪手术组和②单纯脑缺血-再灌注模型组(单纯模型组):2次/d给与5g/LCMC灌胃。③SPC-1组:每日2次给与SPC5mg/100g(5g/LCMC悬浮液)灌胃。④SPC-2组:2次/d次给与SPC10mg/100g(5g/LCMC悬浮液)灌胃。⑤Silybin组:每?BACKGROUND:Silybin has the reactions of anti-free radicals,anti-lipoid perox idation,and anti-lipoid oxidase with extremely low toxicity.However,the effecti veness is affected by its low biological utilization in organisms. Silybin-phos phatidylcholine compound(SPC) has preferably biological utility,and moreover,the phosphatidylcholine in SPC also has anti-free radical and anti-lipoid peroxid ation reaction,and therefore,theoretically,SPC has better pharmacological reacti ons. OBJECTIVE:To understand the protective reaction of SPC in cerebral ischemic re perfusion injury in rats. DESIGN:A complete randomized controlled study. SETTING and PARTICIPANTS:Study was conducted in the Department of Pharmacy of Xinxiang Medical College.Silybin(98%) was obtained from Panjin No.3 Pharmaceut ical Factory of Liaoning Province.SPC(>95%) was synthesized by our department.M DA reagent box was obtained from Nanjing Jiancheng Bioengineering Institute.Inst ruments:SHIMADZU 2550 model Ultraviolet-visible light spectrophotometer,DL-6 m odel low-temperature centrifuge(Wuhan Scientific Instrument Factory).Experiment al animals were obtained from the Experimental Animal Center of Xinxiang Medical College. INTERVENTIONS:Sample medications were given through stomach-perfusion to the rats in each group.The volume of stomach-perfusion was 0.5 mL/100 g.SPC and Sil ybin were suspended separately in 5 g/L of CMC solution①Sham-operation group a nd ②Cerebral ischemic-reperfusion group(model group):stomach-perfusion were c onducted twice a day with 5 g/L of CMC.③SPC-1 group: stomach-perfusion were c onducted twice a day with 5 mg/100 g SPC(5 g/L of CMC suspension).④SPC-2 group :stomach-perfusion were conducted twice a day with 10 mg/100 g SPC(5 g/L of CMC suspension);and⑤Silybin group:stomach-perfusion were conducted twice a day wi th 10 mg/100 g of Silybin(5 g/L of CMC suspension).At 2 hours after the sixth st omach-perfusion(on the third day), surgeries were operated in the rats of each group.100 g/L of urathane was used

关 键 词：水飞蓟 磷脂酰胆碱类 脑缺血 再灌注损伤 

分 类 号：R743[医药卫生—神经病学与精神病学]