壳聚糖在脑组织中的生物相容性(英文)  

Biocompatible features of chitosan in brain tissues

作　　者：周涛[1] 周波[2] 黄书岚[2] 

机构地区：[1]解放军广州军区武汉总医院普外科,湖北省武汉市430070 [2]武汉大学人民医院神经外科,湖北省武汉市430060

出　　处：《中国临床康复》2004年第22期4640-4641,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:壳聚糖在一般组织内的生物相容性已得到证明,但在其脑组织内的生物相容性特征研究及报道较少。目的:探讨壳聚糖在脑组织中的生物相容性,以期为临床提供可用于颅内植入化疗的药物缓释高分子载体。设计:随机对照的实验研究。地点和对象:实验地点:武汉大学第一临床学院实验中心动物手术室。以32只SD大鼠为研究对象,单盲简单随机法分为实验组和对照组。方法:对实验大鼠行开颅手术分别植入壳聚糖和吸收性明胶海绵。观察术后的行为改变和3,7,14,30d时的局部组织反应(苏木精-伊红染色)。主要观察指标:各时期不同材料周围炎性细胞计数。结果:所有动物无明显行为改变,术后3,7,14.30d的组织学观察表明是与吸收性明胶海绵类似的异物反应。术后7d植入物周围3mm区域中性粒细胞、淋巴细胞记数,实验组与对照组比较差异有显著性意义(t=6.98,3.311,P=0.001,0.019);术后14d中性粒细胞、淋巴细胞记数实验组与对照组比较差异有显著性意义(t=6.454,4.899,P=0.001,0.003);术后30d淋巴细胞、纤维细胞记数实验组与对照组比较差异有显著性意义(t=3.767,5.019,P=0.010,0.006)。结论:壳聚糖具有良好的脑组织生物相容性,可安全降解。BACKGROUND:The biocompatibility of chitosan in common tissues has already been proved.However,there are few reports on the biocompatible features of it in bra in tissues. OBJECTIVE:To explore the biocompatibility of chitosan in brain tissues in orde r to provide slow released macromolecule carrier for clinically used chemotherap y. DESIGN:Randomized controled study. SETTING and PARTICIPANTS:Experiment site: Operation room of experiment centre, Wuhan University.Thirty-two SD rats were chosen and divided into experimental g roup and control group by simple random method. METHODS:Chitosan and gelfoam were implanted into rats' skull by operation.Then observed the behavior changes and local histologic reaction in the 3rd,7th,14th and 30th day (haematoxylin-eosin dye). MAIN OUTCOME MEASURES:Inflammatory cells counting of different materials in di fferent stages. RESULTS:There was no obvious behavior change of all rats.The histological reac tions in the 3rd,7th,14th,30th day were similar with the foreign body reaction c aused by gelfoam.There was significant difference on counting of neutrophilic gr anulocyte and lymphocyte between experimental group and control group within the 3 mm area of implanted materials in the 7th day(t=6.98,3.311,P=0.001,0.019).The re was also significance difference on those cell counting between two groups in the 14th day(t=6.454,4.899,P=0.001,0.003).In the 30th day, there was difference on counting of lymphocyte and fibrocyte between two groups(t=3.767,5.019,P=0.01 0, 0.006). CONCLUSION:There is very good biocompatibility of chitosan with brain tissue.I t can be degraded safely.

关 键 词：壳聚糖 生物合成 明胶海绵 吸收性 生物相容性材料 

分 类 号：R74[医药卫生—神经病学与精神病学]