氦氖激光与电针穴位刺激对新生大鼠脑缺血缺氧后海马神经元存活的影响(英文)  

Impacts of He-Ne laser and electric acupuncture on hippocampal neuron survival in cerebral hypoxic ischemia in neonatal rats

作　　者：吴爱群[1] 高艳[2] 刘伟[3] 李宛青[4] 曾光伟[2] 万国兰[2] 

机构地区：[1]解放军第二军医大学解剖学教研室,上海市200433 [2]郑州大学医学院解剖学教研室,河南省郑州市450052 [3]北京协和医科大学人体解剖教研室,北京市100005 [4]郑州师范高等专科学校生命科学系,河南省郑州市450015

出　　处：《中国临床康复》2004年第22期4659-4661,共3页Chinese Journal of Clinical Rehabilitation

基　　金：河南省科技攻关项目(02110438);河南省教育厅攻关项目(023100015)~~

摘　　要：背景:缺血缺氧性脑病(hypoxic-ischemicbraindamage,HIBD)是引起脑性瘫痪的常见病,目前对其治疗尚未有特效的方法。目的:探讨氦氖激光穴位照射与电针穴位刺激两种疗法对新生大鼠脑缺血缺氧后海马神经元存活、发育及其脑源性神经营养因子(brain-derivedneurotrophicfactor,BDNF)、胆碱乙酰基转移酶(cholineacetyltransferase,ChAT)表达的作用,比较与分析两种疗法作用的差异,探讨治疗HIBD的新方法。设计:完全随机设计,实验对照研究。地点与材料:研究的地点为第二军医大学和郑州大学解剖教研室。材料为58只7d龄Wistar大鼠(购自河南省实验动物中心),体质量12～15g,雌雄不拘。干预:随机分为4组:对照组、缺血缺氧组、缺血缺氧后氦氖激光穴位照射组(简称激光组)和缺血缺氧后电针穴位刺激组(简称电针组)。缺血缺氧组～电针组动物参照于晓虹的半球性脑缺血缺氧实验模型法制作左半球缺血缺氧模型,激光组-电针组均选择“大椎”和“百会”穴位分别给予激光照射与电针刺激,22d后取脑作组织切片,HE、Nissl染色以及BDNF和ChAT免疫组织化学染色。主要观察指标:光镜下观察神经元及其尼氏体,检测灰度值与记数ChAT和BDNF阳性细胞以及统计学处理。结果:①对照组海马神经元结构清晰,胞浆内充满深蓝色尼氏体(灰度值122.90±12.BACKGROUND:Hypoxic-ischemic brain damage(HIBD) that often results in cerebral palsy continues to be a common disease in perinatal medicine. At present,no eff ective therapeutic strategies has yet been developed to counteract this conditio n. OBJECTIVE:To study the effect of He-Ne laser acupoint irradiation and electri c acupuncture on hippocampal neuron survival and development as well as the expr essions of brain-derived neurotrophic factor(BDNF) and choline acetyl transfera se(ChAT) in HIBD in neonatal rats.To compare and analysis the different effectiv eness of two treatments in order to explore the new method against HIBD. DESIGN:A completely randomized controlled study. SETTING and PARTICIPANTS:The study was conducted at the Department of Anatomy of the Second Military Medical University of Chinese PLA and Zhengzhou Universit y.Fifty-eight seven-day-old rats were obtained from the experimental animal c enter of Henan Province with a body mass from 12 to 15 g in either gender. INTERVENTIONS: Rats were randomly allocated into four groups: control group(I) that neither ligated nor exposed to hypoxia,HIBD group(II) that exposed to hypox ic-ischemic condition,laser irradiation group(Ⅲ) and electric acupuncture grou p(EA,Ⅳ).Left brain Hypoxic-ischemic injury model were established in all anima ls of Ⅱ-Ⅳ groups on basis of Yu Xiaohong's animal model of unilateral hypoxi a-ischemia in neonatal rats. Animals in group Ⅲ and Ⅳ were treated either w ith He-Ne laser acupoint irradiation(Ⅲ) or EA(Ⅳ) in the next day following hy poxia synchronously by choosing dazhui and baihui acupoints for the treatment.Fi fty-eight rats were subjected to perfusion-fixation 22 days later,the left bra ins were paraffin wax embedded,sectioned and stained by HE and Nissl staining as well as ChAT and BDNF immunohistochemical staining. MAIN OUTCOME MEASURES:The neurons and Nissl bodies were observed,the grey scal e values were measured and the number of ChAT and BDNF positive neurons was coun ted under microscop

关 键 词：激光 电针 低氧-缺血 脑 脑源性神经营养因子 乙酰基转移酶类 

分 类 号：R743[医药卫生—神经病学与精神病学]