P53蛋白、EGFR和P物质在扇贝多肽保护的中波紫外线辐射无毛小鼠皮肤中的表达  

INHIBITORY EFFECTS OF POLYPEPTIDE FROM CHLAMYS FARRERI ON THE EXPRESSIONS OF P53 PROTEIN, EGFR AND SUBSTANCE P IN HAIRLESS MICE SKIN AFTER CHRONIC UVB IRRADIATION

作　　者：张玉江[1] 王美芝[2] 张海平[3] 张岫美[4] 王春波[5] 

机构地区：[1]青岛大学医学院继续教育科,山东青岛266021 [2]青岛大学医学院附属医院神经外科 [3]青岛大学医学院附属医院检验科 [4]山东大学医学院药物研究所 [5]青岛大学医学院药理学教研室

出　　处：《青岛大学医学院学报》2004年第2期128-130,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目 ( 3 9970 63 8);青岛市科技局资助项目 ( 2 0 0 1 2 8 5 0 )

摘　　要：①目的　研究局部应用扇贝多肽 (PCF)对中波紫外线 (UVB)长期辐射无毛小鼠皮肤组织P5 3蛋白、表皮生长因子受体 (EGFR)和P物质 (SP)表达的抑制作用。②方法　随机将昆明种无毛小鼠分为对照组 (Ⅰ组 )、UVB模型组 (Ⅱ组 )、UVB +5 0 g/LPCF组 (Ⅲ组 )、UVB +2 0 0g/LPCF组 (Ⅳ组 )和UVB +1 0 0 g/LVitC组 (Ⅴ组 )。Ⅰ组小鼠背部皮肤涂双蒸水 (每只 1mL/d) ,共 30d。Ⅱ组、Ⅲ组、Ⅳ组和Ⅴ组小鼠背部皮肤分别涂双蒸水、5 0 g/LPCF、2 0 0 g/LPCF和 1 0 0g/LVitC ,每只 1mL/d ,并经UVB辐射 (每天 1次 ,每次剂量为 5 .1 5× 1 0 -2 J·cm-2 ) 30d(总辐射剂量为 1 5 .4 5J·cm-2 )。免疫组化分析法检测无毛小鼠背部皮肤P5 3蛋白、EGFR及SP的表达。③结果　连续UVB辐射可致无毛小鼠皮肤突变型P5 3蛋白、EGFR及SP呈过度表达 ,Ⅱ组与Ⅰ组相比较 ,差异有显著意义 (F =5 4 .2 3～ 6 7.35 ,q =1 1 .2～ 1 9.3,P <0 .0 1 ) ;Ⅲ～Ⅴ组与Ⅱ组比较 ,突变型P5 3蛋白、EGFR及SP表达差异亦有统计学意义 (q =8.9～ 1 0 .5 ,P <0 .0 5 ) ;Ⅳ组与Ⅲ组相比较 ,突变型P5 3蛋白、EGFR及SP的表达均明显减弱 ,差异有统计学意义 (q =1 2 .5～ 1 4 .6 ,P <0 .0 5 )。 ④结论　PCF能够抑制UVB所致P5 3蛋白、EGFR和SP的过表达 。Objective To investigate the inhibitory effects of topically applied polypeptide from Chlamys farreri(PCF) on the expressions of P53, epidermal growth factor receptor(EGFR) and substance P(SP) in hairless mice skin following chronic ultraviolet B irradiation. Methods Hairless mice were randomly divided into five groups(eight in each group): control group, UVB- model group, UVB+50 g/L PCF group, 200 g/L PCF group, and 100 g/L vitamin-C group. Distilling water, PCF, and vitamin C were applied on the backs of the mice (1 mL per mouse a day) in different groups, respectively. The mice were exposed to UVB with a radiant intensity of 5.15×10 -2 J·cm -2 for 30 days (total radiant intensity: 15.45 J·cm -2 ) except those of the control group. Immunohistochemical analysis was employed to examine the expressions of P53, EGFR and SP in the dorsal skin of mice. Results Chronic UVB radiation could induce the overexpressions of mutant P53, EGFR and SP in the skin of hairless mice. The difference between the control group and the UVB-model group was significant ( F=54.23-67.35, q=11.2-19.3, P < 0.01). The expressions of P53, EGFR and SP in the other three groups were decreased than those of the UVB-model group, the differences were also significant ( q=8.9-10.5, P <0.05). The expressions in the 200 g/L PCF group were markedly decreased, and the difference between UVB+50 g/L PCF group and 200 g/L PCF group was significant ( q=12.5-14.6, P < 0.05 ). Conclusion PCF could inhibit the overexpressions of P53, EGFR and substance P and might protect the skin from photocarcinogenesis and photoaging.

关 键 词：扇贝多肽 紫外线 蛋白质 P53 受体 表皮生长因子 P物质 小鼠 近交HRS 

分 类 号：R966[医药卫生—药理学]