珊瑚藻R-藻红蛋白rpeA和rpeB基因全长cDNA克隆与序列分析(英文)  

Cloning and Sequence Analysis of the Full-length cDNAs Encoding rpeA and rpeB of R-Phycoerythrin from Corallina officinalis

作　　者：王盛[1] 钟伏弟[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福州350002

出　　处：《中国生物化学与分子生物学报》2004年第4期428-433,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：福建省科技厅重大项目 (No .2 0 0 0H0 0 4和No .2 0 0 1Z12 7)~~

摘　　要：依据珊瑚藻 (CorallinaofficinalisL .)藻红蛋白rpeA和rpeB的DNA序列 (AF5 1 0 986 )设计引物 ,通过PCR RACE方法扩增得到rpeA和rpeB的cDNA序列 .序列分析表明 ,该序列采用多顺反子转录策略 ,全长 2 2 5 7bp(AF5 42 5 5 4) ,排布顺序为 5′UTR rpeB 间隔区 rpeA 3′UTR .5′非编码区 4 93bp ,rpeB基因 5 34bp ,基因间隔区 1 0 1bp ,rpeA基因 4 95bp ,3′非编码区 6 34bp .在rpeA和rpeB的基因起始密码子上游均存在类似原核核糖体结合的Shine Dalgarno (SD)序列 .在rpeA基因终止密码子下游 1 1 0bp处还存在着一个可能的开放阅读框架 .经检索GenBank发现 。The full length cDNAs encoding rpeA and rpeB from Corallina officinalis L. were cloned with the primers derived from the DNA sequence (AF510986) using RACE method. The sequences of these clones were analyzed, and there were two open reading frames (ORF) that were co transcribed into a 2257 bp polycistronic transcript (AF542554). The structure of this cDNA is arranged as 5′ UTR rpeB intergenic region rpeA 3′ UTR with 493?bp, 534?bp, 101?bp, 495?bp and 634?bp, respectively. Shine Dalgarno (SD) sequences are found both at 13 bp upstream to the initiator of rpeA and rpeB, which are TAAGGAGA for rpeB and TAAGGAAA for rpeA , respectively. According to the ORF finder prediction, another potential ORF was identified at 110?bp downstream to the stop codon of rpeA with the SD like motif at 9 bp upstream to the predicted initiation codon. To the best of our knowledge, this is the first report of the cDNA sequence analysis for rpeA and rpeB of phycoerythrin from the marine red algae.

关 键 词：珊瑚藻 藻红蛋白 快速分离cDNA末端(RACE) 

分 类 号：Q943.2[生物学—植物学]